Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved. Abstracts e257 Objective: Bioactive peptides (BP) have been widely pursued for the management of hypertension showing an angiotensin I converting enzyme (ACE) inhibitory ca- pacity. They have been widely pursued for the management of hypertension and are believed to exert beneficial physiological effects on the endothelium. We aimed to test ACE inhibitory capacity of a protein hydrolysate containing characterized BP from Spanish dry-cured ham and four purified BP (KPVAAP, KAAAATP, KPGRP, AAATP). Secondly, the current study evaluates beneficial changes in protein ex- pression changes and antioxidant activity over the endothelium. Design and method: ACE inhibitory activity of BP was evaluated using an en- zymatic extract of transiently transfected EA.hy 926 endothelial cells. Potential mechanisms of action of BP over endothelial function were quantified by RT-PCR after treatment with TNF- 100 ng/l or H2O2 300 mM w/o BP. Results: Significant ACE inhibition was observed at different concentrations of cured ham hydrolysate and purified BP in a dose-response manner. The following IC 50 were found for each BP: Cured ham hydrolysate 410,34 ± 55,79 mg/ml, KPVAAP 108,79 ± 45,76 M, KPGRP 485,50 ± 43,47 mM, AAATP 1317,32 ± 161,65 mM and KAAAATP 1267,54 ± 43,7516 M. Cured ham hydrolysate at 50 mg/ml promoted antioxidant activity through catalase, superoxide dismutase and eNOS mRNA higher expression in the presence of H2O2 (p = 0.04, p = 0.04 and p = 0.01, respectively). It also prevented an adhesive marker expression such as ICAM-1 in the presence of TNF- (p < 0.001). Moreover, purified AAATP (300 M) also prevented the TNFa- increased ICAM-1 mRNA expression (p = 0.008). KPVAAP and AAATP reverted the TNF- decreased eNOS mRNA expression (p = 0.02 and p = 0.04, respectively). Conclusions: Those results suggest protective effects of biopeptides against inflammation and oxidant agents as well as antihypertensive activity. Bioactive peptides from Spanish dry cured-ham could then display benefits in the cardiovas- cular system, with potential clinical relevance. LB.02.22 DRY-CURED HAM, ITS EFFECTS ON HUMAN BLOOD PRESSURE AND CARDIOVASCULAR RISK: A CLINICAL STUDY S. Montoro Garcia 1 , J. J. Martinez Díaz 2 , J. Abellan Huerta 3 , P. Ramos Ruis 2 , F. G. Clavel Ruiperez 2 , P. Zafrilla 1 , F. Celdran 1 , L. Tejada 1 , J. Abellán Aleman 1 . 1 Cátedra de Riesgo Cardiovascular UCAM, Murcia, Spain, 2 Hospital General Uni- versitario Santa Lucia, Cartagena, Spain, 3 Hospital de Almansa, Albacete, Spain Objective: To investigate whether consuming cured-pork ham with characterized ACE inhibitory activity biopeptides modifies blood pressure and improves risk factors. It is either uncertain whether pork meat with lower proteolysis would also produce an effect on BP. Design and method: A total of 38 participants with systolic BP 125–139 mmHg, age 35-y were recruited and enrolled in a two-arm, crossover, randomised double- blind placebo-controlled randomised trial. Participants received 80 g cured-pork ham of >11 months proteolysis or 100 g placebo (cooked ham) for a 4-week pe- riod followed by a 2-week washout before crossing over to the other treatment for 4-weeks. Risk factors and biomarkers of CVD risk were assessed at baseline and completion of each treatment (4 assessments). Both meat products were analysed in order to confirm the biopeptide presence. Blood pressure was measured with 24 h ambulatory monitoring. Blood lipid profile, plasma glucose and creatinine were measured. 24-h urine collection at 4 timepoints was analysed to account for potential confounding of urinary sodium excretion (intake). Results: The consumption of dry-cured ham did not affect sodium excretion despite a 3.75 g daily higher intake (p = 0.78). Besides, total cholesterol, LDL and basal glucose dropped after dry-cured ham intake (p = 0.00019, p = 0.021 and p = 0.014, respec- tively), while the rest of biochemical markers remained unaltered. Cooked ham did not affect any of biochemical markers. No significant changes were found in average, day-time or night-time systolic and diastolic pressures for any of the meat products. Conclusions: A daily intake of 80 g dry-cured meat did not increase SBP/DBP nor 24 h sodium excretion Dry-cured ham biopeptides could exert a plethora of activities over the cardiovascular system including lipid and glucose metabolism. Additional studies are needed to confirm the effects of dry-cured meat biopeptides on diverse risk factors in pathological conditions. LB.02.24 PREVALENCE OF PHYSICAL ACTIVITY AND METABOLIC SYNDROME IN POPULATION OF INDUSTRIAL WORKERS IN OLSZTYN, POLAND N. Kwella 1 , M. Szczubelek 2 , P. Zielecki 2 , J. Harazny 2,3 , T. Stompór 1 . 1 Department of Nephrology and Hypertension, University of Warmia nad Mazury, Olsztyn, Poland, 2 Department of Pathophysiology, University of Warmia and Mazury, Olsztyn, Poland, 3 Clinical Research Center, Department of Nephrology and Hypertension, University Erlangen-Nuremberg, Olsztyn, Poland Objective: To evaluate the prevalence of physical activity and metabolic syn- drome in population of industrial workers in Olsztyn, Poland. Design and method: N = 173 subjects (62 women, 48 ± 9 years old; 111 men, 45 ± 10 years old, p = 0.12) employed in the same industrial workplace. Analyses were performed separately for 2 groups depending on physical activity: ACT+ (subjects that do sports or gymnastics at least 30 minutes 3 times a week) and ACT- (do sport/gymnastics less than 30 minutes 3 times a week or never). Sub- jects working physically who fulfill ACT- criteria were excluded from analysis (n = 23). Metabolic syndrome was diagnosed according to the IDF 2005 guide- lines. Blood pressure was measured according to the ESH/ESC guidelines. Blood and urine laboratory tests (serum glucose, triglycerides, HDL-, LDL- and total cholesterol, serum creatinine) were measured in a certified laboratory. Results: 52.3% of study group meet the ACT+ criteria (M: 54.5%, F:50.9%). MS was diagnosed in 35.4% subjects (M:43.0%, F:21.1%, p = 0.003). The preva- lence of MS in ACT- was significantly higher in men than in women (50.0% vs 15.4%, p = 0.004) and no significant difference was found in ACT+ (34.0% vs 17.2%, p = 0.109). In ACT+ waist circumference, pulse pressure was significantly lower than in ACT- (respectively 95 ± 10 vs 101 ± 11 cm, p = 0.013, 44 ± 11 vs 48 ± 10 mmHg, p = 0.016) and total cholesterol was significantly higher than in ACT- (221 ± 43 vs 203 ± 28 mg/dl, p = 0.03). Conclusions: The prevalence of metabolic syndrome in population of industrial workers in Olsztyn, Poland is much higher than an average in Poland (35.4% vs 28.6%), mainly in men (43% vs 31%). MS was diagnosed more frequently in men than in women. The most common MS criterion fulfilled by the subjects was DPB. In men ACT+ MS was less frequent than in men ACT-. It was not observed in women. LB.02.26 ASSOCIATION OF SERUM URIC ACID AND ENDOTHELIAL DYSFUNCTION IN PATIENTS WITH METABOLIC SYNDROME C. Singh 1 , S. Jain 1 , V. Dhawan 2 , N. Kalra 3 , S. Kumari 1 . 1 Post Graduate Institute of Medical Education and Research- Department of Internal Medicine, Chandigarh, India, 2 Post Graduate Institute of Medical Education and Research- Department of Experimental Medicine, Chandigarh, India, 3 Post Graduate Institute of Medical Education and Research- Department of Radiodiagnosis, Chandigarh, India Objective: Hyperuricemia occurs commonly in patients with metabolic syn- drome. However, its role in the pathogenesis of metabolic syndrome and endothe- lial dysfunction is unclear. Through this study, we aimed to study the association of endothelial dysfunction with uric acid levels in patients of metabolic syndrome. Design and method: 71 patients of Metabolic Syndrome (International Diabetes Federation definition) were included in the study. Anthropometric measurements, serum uric acid levels, fasting blood sugar levels and lipid levels were measured after an 8 hour fasting period. Serum reactive nitrogen intermediates (RNI) were measured using Griess reagent. Flow mediated vasodilation (FMD) of the brachial artery was measured and endothelial dysfunction was defined as an increase in diameter < 10% post compression. Results: Amongst the 71 patients (41 females, 30 males, 88.7% hypertensive, 43.7% diabetic), mean age was 50.8 ± 10.6 years, BMI was 28.6 ± 4.86 kg/m 2 , waist circumference was 99.5 ± 9 cms, triglyceride was 218.4 mg/dl, HDL was 45.6 ± 13.9 mg/dl, LDL was 125.7 mg/dl and cholesterol was 200.3 mg/dl. Mean serum uric acid was 5.32 ± 1.44 mg/dl. 41 patients (57.7%) had an impaired FMD (iFMD) and had a significantly higher serum uric acid level than patients with normal FMD (p = 0.047). A cut off value of 4.75 mg/dl for serum uric acid was calculated after formulating an ROC curve. Patients with a higher value than the cut off had an increased prevalence of iFMD which was statistically significant (p = 0.012). RNI levels were not significantly associated with serum uric acid lev- els and presence of iFMD (p = 0.169).