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Effect of Harmonic Imaging Without Contrast on Image
Quality of Transesophageal Echocardiography
Guido Rocchi, MD, Nico de Jong, PhD, Tjebbe W. Galema, MD,
Jaroslaw D. Kasprzak, MD, PhD, and Folkert J. Ten Cate, MD, PhD
H
armonic imaging (HI) has been developed to
improve the potential of contrast echocardiogra-
phy.
1,2
It exploits the fact that microbubbles can res-
onate when hit by ultrasound producing harmonics as
multiples of transmitted frequency.
3–5
If the ultra-
sound machine is tuned to receive a second harmonic
frequency selectively, it can differentiate contrast
from tissue.
6
However, baseline imaging is not deleted
completely, because of nonlinear backscatter proper-
ties of tissue.
7,8
This allows one to use harmonic
imaging without contrast agents; this modality has
been called tissue HI or native HI. Recent transtho-
racic echo studies have shown the ability of tissue HI
to improve endocardial border delineation.
9 –11
No
previous study has been performed to assess the role
of tissue HI during transesophageal echocardiography
(TEE). Usually, TEE shows high-quality images, but
at several occasions, as during cardiac surgery when
the heart is dislocated from its normal position, image
quality can be suboptimal. We developed a prototype
transesophageal transducer that is able to obtain HI,
and compared it with TEE image quality of harmonic
and fundamental (conventional) imaging. The study
was performed in the operating room during coronary
artery bypass surgery (CABG).
•••
Fourteen consecutive patients (mean age 60 6 8
years) referred for CABG were enrolled in the study.
Transesophageal echocardiograms in both harmonic
and fundamental modes were performed before and
immediately after CABG in the operating room. The
second acquisition was performed just after termina-
tion of cardiopulmonary bypass pump to evaluate
possible new wall motion abnormalities before its
removal. A prototype transesophageal monoplane
transducer interfaced with a Vingmed System Five
ultrasound machine (Vingmed, Oslo, Norway) was
used for all studies. Transgastric short-axis and esoph-
ageal 4-chamber views were obtained in all patients.
Fundamental images were obtained using a 4.4-MHz
transmitted frequency, whereas for the harmonic
mode the transducer transmitted at 2.9 MHz and re-
ceived at 5.8 MHz. The 64-element broad-band trans-
ducer received wide band in fundamental and small
band in the second harmonic mode. Mechanical index
was set at 1.0. Imaging was optimized by increasing
dynamic range or by adjusting overall gain. Dynamic
range was set at .60 dB in all cases. In 5 patients an
additional second transducer with a higher fundamen-
tal frequency (5.7 MHz) was tested after CABG to
compare HI with fundamental imaging obtained by a
commercial probe (Vingmed). Two experienced ob-
servers, blinded for the acquisition method used,
scored each echocardiogram for visualization of en-
docardial borders. Visibility of endocardium was de-
fined segment by segment using the following score:
0 5 not visible; 1 5 incomplete visualization during
the all cardiac cycle; 2 5 incomplete visualization
during part of the cardiac cycle; and 3 5 complete
visualization. Wall motion was scored in a 4-grade
model: 1 indicating normal wall motion, 2 hypokine-
sia, 3 akinesia, and 4 dyskinesia.
Inter- and intraobserver variability for visibility of
endocardial borders was scored by 2 independent ob-
servers and 30 days later by the first observer. Both
observers were blinded for the acquisition method
used. Kappa coefficients were calculated using a SAS
system (SAS Institute, Cary, North Carolina) A k
coefficient of .0.4, .0.6, and .0.8 indicated fair,
good, and excellent agreement, respectively. Data are
presented as mean 6 1 SD. Differences between fun-
damental and harmonic score were evaluated by anal-
ysis of variance for repeated measures or by paired
Student’s t test when appropriate. A p value ,0.05
was considered significant.
In all, 168 segments (12 segments 3 14 patients)
were scored at both harmonic and fundamental imag-
From the Heartcentre, Erasmus University, Rotterdam, The Nether-
lands. Dr. Rocchi was supported by the Institute of Cardiology, Univer-
sity of Bologna, Italy. Dr. Kasprzak was supported by an educational
grant of the European Society of Cardiology, Nice, France. Dr. Ten
Cate’s address is: Department of Cardiology, ZuiderZiekenhuis,
Groene Hilledijk 315, 3075EA Rotterdam, The Netherlands. E-mail:
f.ten.cate@zuiderziekenhuis.nl. Manuscript received February 1,
1999; revised manuscript received June 14, 1999, and accepted
June 15.
1132 ©1999 by Excerpta Medica, Inc. All rights reserved. 0002-9149/99/$–see front matter
The American Journal of Cardiology Vol. 84 November 1, 1999 PII S0002-9149(99)00521-4