https://doi.org/10.1177/1120672119874947
European Journal of Ophthalmology
1–5
© The Author(s) 2019
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DOI: 10.1177/1120672119874947
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Ophthalmology
Introduction
A normal cornea is avascular; however, under certain condi-
tions capillaries may invade from the limbal vascular plexus
causing corneal neovascularization. A wide variety of insults
including inflammations, corneal ulcers, ischemia, degenera-
tions, contact lenses, trauma, limbal stem cell deficiency, and
chemical/ thermal burns can cause corneal neovasculariza-
tion.
1,2
Corneal neovascularization may not only significantly
decrease visual acuity, but also may increase the chance of
graft rejection after penetrating keratoplasty (PKP).
3–5
Depending on the time of onset and chronicity, there are
multiple strategies for the treatment of corneal neovascu-
larization. Medical treatment modalities include steroids,
non-steroidal anti-inflammatory agents, cyclosporine,
bevacizumab, ranibizumab, tacrolimus, TNFα inhibitors,
heparin, and so on.
2,6
None of them is free of adverse
effects and because of poor compliance, patients may dis-
continue their use.
Regression of corneal
neovascularization: Adiponectin
versus bevacizumab eye drops
Alireza Baradaran-Rafii
1
, Azin Ashnagar
1
,
Saeed Heidari Keshel
2
, Sayena Jabbehdari
3
and Ghazaleh Baradaran-Rafii
4
Abstract
Purpose: Comparing the effect of adiponectin versus bevacizumab in decreasing corneal neovascularization.
Methods: This study was conducted on 30 eyes of 30 New Zealand Albino male rabbits. Corneal neovascularization was
induced by a single 7-0 silk suture 2 mm long and 1 mm in front of the limbus for 2 weeks. Rabbits were randomly divided
into three groups of adiponectin (20 µg/mL), bevacizumab (5 mg/mL) and artificial tears. The treatments continued up
to 14 days.
Results: At the end of 14 days, the average length of vessels in rabbits treated with adiponectin, bevacizumab and
control groups decreased from 2.12 ± 0.32 mm to 0.89 ± 0.46 mm (57.68% ± 19.98%) (P < 0.001), 2.30 ± 0.41 mm to
1.30 ± 0.58 mm (42.49% ± 27.17%) (P = 0.048) and from 2.12 ± 0.44 mm to 1.81 ± 0.42 mm (14.81% ± 5.64%) (P = 0.112),
respectively. The length of vessels decreased 57.68% ± 19.98% and 42.49% ± 27.17% in adiponectin versus bevacizumab
groups, respectively (P = 0.527). The average surface area of vessels in rabbits treated with adiponectin, bevacizumab
and control groups reduced from 5.02 ± 1.50 mm
2
to 1.40 ± 0.75 mm
2
(70.64% ± 17.76%) (P < 0.001) 0.34 ± 1.1 mm
2
to 2.80 ± 1.04 mm
2
(48.24% ± 19.23%) (P = 0.039) and 5.12 ± 2.92 mm
2
to 4.4 ± 2.55 mm
2
(14.68% ± 4.19%) (P = 0.117).
Mean surface area of vascularization decreased 70.64% ± 17.76% and 48.24% ± 19.23% in adiponectin versus bevacizumab,
respectively (P = 0.013).
Conclusions: The results of this study suggest that topical adiponectin can decrease recent corneal neovascularization.
Keywords
Bevacizumab, adiponectin, corneal neovascularization, animal model
Date received: 7 March 2019; revised: 4 August 2019; accepted: 17 August 2019
1
Ocular Tissue Engineering Research Center, Shahid Beheshti
University of Medical Sciences, Tehran, Iran
2
Proteomics Research Center, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
3
Department of Ophthalmology and Visual Sciences, University of
Illinois at Chicago, Chicago, IL, USA
4
School of Civil Engineering, College of Engineering, University of
Tehran, Tehran, Iran
Corresponding author:
Azin Ashnagar, Ocular Tissue Engineering Research Center, Shahid
Beheshti University of Medical Sciences, No. 23, Paidarfard St., Boostan
9 St., Pasdaran Ave, Tehran, 16666, Iran.
Email: azin_ashnagar2@yahoo.com
874947EJO 0 0 10.1177/1120672119874947European Journal of OphthalmologyBaradaran-Rafii et al.
research-article 2019
Original research article