RESEARCH ARTICLE 10 Open Access: http://ijaam-unud.org Liya Rosdiana, Wimpie Pangkahila, I Gusti Made Aman (Administration of gallus domesticus boiled chicken white egg.....) 1 Master Program in Biomedical Sciences Study Program Specificity of Anti-Aging Postgraduate Program Udayana University Denpasar 2 Department of Andrology and Sexology, Faculty of Medicine, Udayana University 3 Department of Pharmacology, Faculty of Medicine, Udayana University Submitted: 17 December 2018 Accepted: 28 February 2019 Published: 1 June 2019 Indonesian Journal of Anti-Aging Medicine Volume 3, Number 1, January-June 2019 : 10-14 Administration of gallus domesticus boiled chicken white egg increased amh levels of premenopausal female wistar rats Liya Rosdiana 1* , Wimpie Pangkahila 1,2 , I Gusti Made Aman 1,3 ABSTRACT Introduction: Aging is a complex multifactorial process and leads to functional changes one of which is the decline in reproductive function. Decrease in reproductive function in women in in the form of decreased ovarian reserve can be detected by low levels of Anti_mullerian Hormone (AMH). This study aims to prove the effect of administration of Gallus domesticus boiled chicken white egg increased AMH levels of premenopausal female wistar rats. Methods: This research was experimental animal with Pretest-Posttest Control Group Design. The study conducted on 16 premenopausal female wistar mices, aged 17-18 months, weighing 250-260 grams. The mice were divided into 2 goups: the control group (placebo), which was given gelatin flour by 7.3 gram 3 times daily for 4 weeks and the group treted with Gallus domesticus boiled chicken white egg by 7.3 gram 3 times daily for 4 weeks by oral. Blood AMH level were measured before and after treatment for both group. Results: The result showed that before treatment, mean AMH level both in control group and treatment group were not significantly different (p > 0,05). After treatment, mean AMH level both in control group and treatment group were significantly different (p < 0,05). The administration of Gallus domesticus boiled chicken white egg increased AMH levels by 0.804 ng/ml. Conclusion: It can be concluded that administration of Gallus domesticus boiled chicken white egg increased AMH level. The mean AMH level after treatment had a significant difference between control group (placebo) and treatment group with p = 0.001. Keywords: Anti-Mullerian Hormone, Aging, Gallus domesticus boiled chicken white egg *Corresponding Author: Liya Rosdiana; Master Program in Biomedical Sciences Specificity of Anti-Aging; Postgraduate Program Udayana University Denpasar; liarose.dr@gmail.com INTRODUCTION Every creature in this world will definitely experience the aging process. Physiologically, aging will cause changes in function and structure at both the cellular level, the organ and the body system. Aging is not only caused by the age factor but also influenced by various other factors such as internal factors such as free radicals, hormone deficiency, glycosylation process, methylation, apoptosis, decreased immune system and genes, as well as external factors such as unhealthy lifestyles, healthy, bad habits, environmental pollution, stress and poverty 1 . Aging can be known by seeing signs or changes that occur that can not only be seen physically but also anatomically, biochemically and molecularly. e signs of aging are also closely related to the function of various organs of the human body in supporting daily life 1 . One of the aging processes women will face is a gradual decrease in estrogen and progresterone hormones that cause aging of the reproductive organs as well as the weakening of ovarian function. At this time, the ovaries are no longer sensitive to stimuli from the brain, so the eggs cannot grow until they ripen so rarely ovulate and eventually stop. Today, many women who experience early menopause and aging reproductive organs before the age that should be. Various researchers suggest that early menopause occurs in productive women of less than 40 years of age 2,3 . Based on the report of the Association of Endocrinology of Reproduction and Fertility of Indonesia (HIFERI) and In Vitro Indonesia Fertilization Association (PERFITRI) (2013), the percentage of female infertility in Indonesia indicates that many Indonesian women are experiencing aging problems in reproductive organs, thus experiencing infertility problems especially at young age 4 . Meanwhile, fertility is a vital aspect for women in carrying out their life. erefore, it is important to make efforts to prevent,