~ 1736 ~
International Journal of Chemical Studies 2019; 7(2): 1736-1739
P-ISSN: 2349–8528
E-ISSN: 2321–4902
IJCS 2019; 7(2): 1736-1739
© 2019 IJCS
Received: 24-01-2019
Accepted: 26-02-2019
S Dash
Ph.D. in Veterinary
Gynaecology and Obstetrics.
Department of Veterinary
Gynaecology and Obstetrics,
Faculty of Veterinary and
Animal Sciences, West Bengal
University of Animal & Fishery
Sciences Kshudiram Bose Sarani,
Kolkata, West Bengal, India
S Basu
Professor, Department of
Veterinary Gynaecology and
Obstetrics, Faculty of Veterinary
and Animal Sciences, West
Bengal University of Animal &
Fishery Sciences, Kshudiram
Bose Sarani, Kolkata, West
Bengal, India
PC Mishra
Professor, Department of
Veterinary Gynaecology and
Obstetrics, Faculty of Veterinary
and Animal Sciences, West
Bengal University of Animal &
Fishery Sciences, Kshudiram
Bose Sarani, Kolkata, West
Bengal, India
K Ray
Professor, and Head,
Department of Veterinary
Gynaecology and Obstetrics,
Faculty of Veterinary and
Animal Sciences, West Bengal
University of Animal & Fishery
Sciences, Kshudiram Bose
Sarani, Kolkata, West Bengal,
India
Correspondence
S Dash
Ph.D. in Veterinary
Gynaecology and Obstetrics.
Department of Veterinary
Gynaecology and Obstetrics,
Faculty of Veterinary and
Animal Sciences, West Bengal
University of Animal & Fishery
Sciences Kshudiram Bose Sarani,
Kolkata, West Bengal, India
Effect of immunomodulators on certain
haematological parameters in ameliorating bovine
Endometritis
S Dash, S Basu, PC Mishra, K Ray
Abstract
In the present investigation seventy two endometritic cows selected from some parts of Odisha and West
Bengal after meticulous screening and were allotted randomly into six equal groups (n=12). Group I, II
and III animals were administered with 100μg of E. coli LPS (LPS), 500 mg of oyster glycogen (OG),
and 1000 mg enrofloxacin through i.u route after reconstituted with 30 milliliter of Phosphate Buffer
Saline (PBS). Oral herbal drugs were used in group IV animals. In group V, 50 ml of normal saline was
infused i.u. Endometritis animals receiving no treatments were considered as control for the study. Blood
sample was collected from experimental cows prior to institution of therapy, 24 hrs after treatment and at
next Estrus for evaluation of different haematological parameters. Use of immunomodulators improved
Hb concentration Significantly (p < 0.05) at post treatment succeeding estrous. A significantly lower (p <
0.05) mean TLC value was observed at succeeding estrus in group I and II than other treatment groups.
mean Nutrophil value at 24 hrs of treatment increased significantly (p < 0.05) from pretreatment value
and it decreased significantly at the next succeeding etrous.
Keywords: Endometritis, cows, immunomodulators, haematology
Introduction
Repeat breeding is one of the major problems affecting reproductive efficiency. The repeat
breeding syndrome is a major source of economic waste and poor reproductive performance in
dairy herds. The presence of clinical and Subclinical Endometritis (SE) is one of the
etiological factors of repeat breeding syndrome. Endometritis is termed as the inflammation of
endometrium and underlying glandular tissue without any systemic signs, occurring at least 21
days after calving (Sheldon et al., 2006)
[1]
. Uterine infections are observed following twin
births, dystocia and surgical interventions employed to aid in cases of dystocia, retention of
fetal membrane, stillbirth, prolapse of uteri, artificial insemination, copulation, and the use of
intrauterine irritants. The inflammatory and immune response to uterine bacterial infection
compromises animal welfare as well as cause subfertility and infertility (Sheldon et al., 2004)
[2]
. Dysfunction of cell immunity coexisting with subclinical Endometritis may be the main
factor causing advanced inflammation of the uterus. There are substantial changes in
subpopulations of immune cells in cows with subclinical Endometritis. Local mechanisms of
uterine immunity vary from general immune mechanisms, the evidence of which are
differences in the percentages of leukocyte subpopulations between uterus and peripheral
blood of cows and possession of a lower phagocytic activity of uterine phagocytes than blood
phagocytes. Knowledge of immunological mechanisms in the uterus of cows during
subclinical Endometritis should enable more precise diagnosis of uterine inflammatory
reactions and may be helpful in choosing proper adjuvant therapy with Immunomodulating
agents. Immunomodulators are the new approach in treating Endometritis. They play their role
to minimize infection by enhancing both systemic and local immunity through proliferating
Certain body components lymphocytes, neutrophils, monocytes, humoral antibodies and PMN
cells (Singh et al., 2001)
[3]
Materials and Method
A total number of seventy two Endometritis affected cows were selected after screening and
were allotted randomly into six equal groups having 12 animals in each group. Group I and II