_____________________________________________________________________________________________________ ++ Ph.D. Scholar; # Professor; † Assistant Professor *Corresponding author: E-mail: rmritunjay602@gmail.com; Int. J. Plant Soil Sci., vol. 35, no. 16, pp. 33-39, 2023 International Journal of Plant & Soil Science Volume 35, Issue 16, Page 33-39, 2023; Article no.IJPSS.101033 ISSN: 2320-7035 Studies on Genetic Divergence of Parental Lines and Their F 1 Progeny in Bottle Gourd [Lagenaria siceraria (Mol.) Standl.] Mritunjay Rai a++* , C. N. Ram a# , Anil Kumar a† , Aastik Jha a† and Ashish Kumar Singh a† a Department of Vegetable Science, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (U.P), India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJPSS/2023/v35i163127 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/101033 Received: 01/04/2023 Accepted: 05/06/2023 Published: 19/06/2023 ABSTRACT The current experiment was carried out in Randomized Complete Block Design with three replications. In present investigation forty-three genotypes (10 lines + 3 testers + 30 F 1 ’s) of bottle gourd were grouped into seven distinct non over lapping clusters. This suggested that the genotypes had a significant amount of variability. Additionally, it was discovered that the genotypes belonging to the same geographic area were clustered together. Thus, there was no consistent relationship between genetic divergence and geographical distribution. Number of genotypes per cluster ranged from fifteen to one. The cluster II was the largest with fifteen genotypes followed by Original Research Article