Research Article
In Vivo Antihypercholesterolemic Potential of
Swietenia mahagoni Leaf Extract
Naveen Yelaware Puttaswamy
1
and Asna Urooj
2
1
Department of Biochemistry, Pooja Bhagavath Memorial Mahajana PG Center, Mysore, India
2
Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangothri, Mysore, India
Correspondence should be addressed to Asna Urooj; asnau321@gmail.com
Received 31 July 2016; Revised 15 September 2016; Accepted 18 September 2016
Academic Editor: Patrizia M. Tarugi
Copyright © 2016 N. Yelaware Puttaswamy and A. Urooj. is is an open access article distributed under the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
e present investigation aims to evaluate antihypercholesterolemic potential of Swietenia mahagoni leaf aqueous extract (MAE)
in diet-induced hypercholesterolemic rat model. In the study, Wistar albino rats (170–220 g) were segregated into 5 groups; all the
groups except normal control group were given high fat diet to induce hypercholesterolemia. Aſter induction of cholesterolemia,
normal control and positive control groups were treated with saline, statin group was treated with atorvastatin, and remaining
two groups received MAE in two doses (250 and 500 mg kg
−1
BW) for a treatment period of one month. Aſter the treatment
period, weight of rats was recorded and they were anesthetized and decapitated. Blood samples were taken and triglycerides, total
cholesterol, LDL-C, HDL-C, malondialdehyde (MDA), and urea were determined. Liver and kidney were taken for the estimation
of lipid peroxides. e positive control group showed higher values of triglycerides (109 ± 5.1 mg/dL), total cholesterol (134 ±
4.6 mg/dL), LDL-C (44 ± 1.2 mg/dL), MDA, and bile acid content when compared to a normal control group (triglycerides (89 ±
3.2 mg/dL), total cholesterol (72 ± 3.4 mg/dL), and LDL-C (28 ± 1.2 mg/dL)). Treatment with MAE decreased the cholesterol levels,
HDL-C, ALT, AST, and bilirubin levels and the effect was dependent on the dose. e results of this study indicated that MAE
possesses hypolipidemic potential and thus could be useful in the treatment of hypercholesterolemic condition.
1. Introduction
Hypercholesterolemia, also known as dyslipidemia, is a
pathological condition which is characterized by elevated
amounts of cholesterol and lipoproteins in blood plasma [1].
Since the condition may result in various metabolic disorders
such as cardiovascular diseases and diabetes, it is one of
the major socioeconomic problems and is the result of a
combination of a modern lifestyle and dietary habits [2]. On
hypercholesterolemic condition, elevated levels of low density
lipoproteins are deposited in the subendothelial region of
arteries leading to inflammation and building up of plaque
ultimately resulting in hypertension, decreased functional
potential of metabolic organs (liver and kidney), and also dia-
betes [3]. Clinical trials conducted on hypercholesterolemic
subjects indicate that the drug which reduces the lipid levels
has significant positive effects on morbidity and mortality [4].
Improving the elevated levels of LDL cholesterol has been
found to reverse the atherosclerosis and hamper progression
of cardiovascular diseases [5, 6]. Dyslipidemia is also a
common condition associated with uncontrolled type II DM
[7].
Swietenia mahagoni is a medicinal plant used by folklore
as a primary health care need around the globe [8]. e
medicinal plant has been widely investigated for various
pharmacological effects [9–12]. In our laboratory, the plant
has been extensively investigated for its phytochemical com-
position, antioxidant potential, and antidiabetic potential by
various in vitro and in vivo assays [13, 14]. In our previous
study in diabetic animal model we have found that along
with glucose lowering ability the extract has potential lipid
lowering ability [15]. e present study hypothesis was based
on the results of previous experiments. With the background,
the study aims to evaluate antihypercholesterolemic potential
of Swietenia mahagoni leaf aqueous extract (MAE) in the diet-
induced hypercholesterolemic rat model.
Hindawi Publishing Corporation
Cholesterol
Volume 2016, Article ID 2048341, 6 pages
http://dx.doi.org/10.1155/2016/2048341