Borneo Journal of Pharmacy https://journal.umpr.ac.id/index.php/bjop/article/view/1830 Vol 6 Issue 1 February 2023 DOI: https://doi.org/10.33084/bjop.v6i1.1830 Pages 22 – 30 e-ISSN: 2621-4814 How to cite: Triadisti N, Zamzani I. Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves. Borneo J Pharm. 2023;6(1):22-30. doi:10.33084/bjop.v6i1.1830 INTRODUCTION Oxidative stress is represented by elevated levels of reactive oxygen species (ROS), which impairs the balance of intracellular reduction-oxidation, and this is seen in a wide variety of diseases 1 . Reactive oxygen species (ROS) such as hydroxyl radicals (OH), singlet oxygen (O2), hydrogen peroxide free radicals (H2O2), ozone (O3), and organic peroxides (ROOH) are thought to induce oxidative stress 2 . Oxidative stress occurs due to a lack of balance between reactive oxygen species (ROS) and antioxidant defenses 3 . This imbalance is a major cause of various diseases today, including cardiovascular disease and diabetes. It is also known to exacerbate DNA mutation and damage, cell damage, aging, neurodegenerative diseases, play a role in oncogenesis, and many other digestive diseases, including ulcers and gastrointestinal disorders 4 . Antioxidants are believed to be important in dealing with oxidative stress caused by ROS. Various synthetic antioxidants are available primarily in the food and beverage industry to preserve and prolong product stability, but these are often Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves Nita Triadisti 1* Irfan Zamzani 2 1 Laboratory of Pharmacognosy- Phytochemistry, Universitas Muhammadiyah Banjarmasin, Barito Kuala, South Kalimantan, Indonesia 2 Department of Pharmacy, Universitas Muhammadiyah Banjarmasin, Barito Kuala, South Kalimantan, Indonesia * email: triadisti@umbjm.ac.id Keywords: Antioxidant DPPH Fractionation FRAP Passiflora foetida Abstract Available synthetic antioxidants have been reported to have mutagenic and toxic effects. On the other hand, natural antioxidants show their superiority as they are not or less toxic. Passiflora foetida has the potential as an antioxidant, but the investigation of the antioxidant activity of the P. foetida chromatography column fraction has not been reported. This studied aims to investigate the antioxidant activity of the column chromatographic fractions of P. foetida leaves. An antioxidant assay using the DPPH and FRAP methods. The extraction was carried out by graded maceration, then fractionation using column chromatography. The antioxidant activity test was carried out using the DPPH and FRAP methods. Thin layer chromatography analysis was performed to determine the chromatogram pattern. The EC50 using DPPH method from n- hexane extract: 129.035 μg/mL, ethyl acetate extract: 206.398 μg/mL, methanol extract: 97.453 μg/mL; while the EC50 using FRAP method from n-hexane extract: 67.851 μg/mL, ethyl acetate extract : 68.981 μg/mL, and methanol extract: 58.787 μg/mL. Column chromatography fractions have antioxidant activity, with FMetPF6 as the fraction with the best activity, with percent inhibition 41.85±1.96 at concentration 25 μg/mL (DPPH), and with percent antioxidant activity 26.03±0.84 at concentration 9 μg/mL (FRAP). Passiflora foetida leaves have great potential as an antioxidant; both the extract and its fractions have antioxidant activity. The FMetPF6 has the best activity compare to other extracts and fractions. Further analysis to determine the various compounds in FMetPF6 using LC- MS/MS will facilitate the active compound's isolation. Received: November 19 th , 2020 1 st Revised: March 13 th , 2021 2 nd Revised: June 22 nd , 2021 3 rd Revised: July 26 th , 2022 Accepted: February 26 th , 2023 Published: February 28 th , 2023 © 2023 Nita Triadisti, Irfan Zamzani. Published by Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya. This is an Open Access article under the CC-BY-SA License (http://creativecommons.org/licenses/by-sa/4.0/). DOI: https://doi.org/10.33084/bjop.v6i1.1830 Research Article