Journal of Biotechnology and Bioengineering Volume 5, Issue 2, 2021, PP 12-21 ISSN 2637-5362 DOI: https://doi.org/10.22259/2637-5362.0502002 Journal of Biotechnology and Bioengineering V5 ● I2 ● 2021 12 Role of Enzymes in Piglets Julijana TOMOVSKA 1 , Kristina VELKOVA 2 1 University, St. KlimentOhridski, -Bitola; Faculty of Biotechnical Sciences-Bitola Partizanska bb 7000 Bitola, R.N.Macedonia 2 Bitolska laboratorija-Medical laboratory in Bitola, R. N. Macedonia *Corresponding Author: Julijana TOMOVSKA, 1University ,,St. KlimentOhridski,, -Bitola; Faculty of Biotechnical Sciences-Bitola Partizanska bb 7000 Bitola, R.N.Macedonia INTRODUCTION Enzymes are a special class of proteins that catalyze chemical reactions in biological systems, in the human body, animals and plants, a whole range of metabolic processes of decomposition and synthesis takes place (Dzekova, S., 2006). Parameters for testing the function of the liver are the following enzymes: ALT-alanine aminotransferase, AST-aspartate aminotransferase, GT-gamma glutamyl transferase, and others. In the liver, ALT catalyzes the transfer of α-amino nitrogen from alanine to α-ketoglutarate forming pyruvate, which is used in gluconeogenesis (Kaneko, J.J., et al., 2008). ALT is found mainly in the liver, but also in smaller amounts in the kidneys , heart, muscles, and pancreas . ALT was formerly called serum glutamic pyruvic transaminase (SGPT). ALT catalyzes the reversible transfer of the amino group from glutamate to pyruvate while replacing the amino group of glutamate with a carbonyl group, reaction 1. Reaction of ALT Catalyzes AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health.AST formerly was called serum glutamic oxaloacetic transaminase (SGOT).Aspartate transaminase catalyzes the interconversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Both ALT and AST levels can test for liver damage, reaction 2. ABSTRACT The purpose of this research is to determine the impact of whey on transaminases, which are indicators of liver disease. For this purpose, two groups of piglets were fed with different amounts of whey (ad libidum and controlled quantity) in the period of 45 days. Every 15 days values were tested for ALT, AST and ɣ -GT serum of piglets, and these parameters were examined in vitro with pathological and normal control serum. From several samples of whey investigated total protein and albumen, as minerals Fe, K, Ca and P, using a spectrophotometric method. In piglets fed by whey, a significant reduction is obtained in the level of transaminases (p <0.05) the lowest values and the smallest deviation values were obtained in the group of piglets which were fed ad libidum. The results in vitro show that whey performed inhibition on the activity of these enzymes ALT 10,71%, AST 8,51% and ɣ -GT from 18.16% in pathological serum, in serum with normal values, whey performed inhibition on ALT for 39.33%, AST for 29.08% and ɣ -GT for 39.59%. Whey impacts on the reduction of transaminases and performs inhibition of enzyme activity in the in vitro test. Keywords: ALT, AST, ɣ -GT, piglets, whey, enzymes in vivo, enzymes in vitro.