179 Phytochemical Screening and Toxicity Test of Various Extracts from Microalgae Dunaliella salina Yosi Yananda Sijabat* , Wilis Ari Setyati, and Retno Hartati Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50275. Indonesia ARTICLE INFO Received: June 01, 2023 Accepted: August 03, 2022 Published: August 17, 2022 Available online: January 29, 2023 *) Corresponding author: E-mail: yosiyanandas@gmail.com Keywords: D. salina A. salina Phytochemical Compounds BSLT 24-hours LC50 This is an open access article un- der the CC BY-NC-SA license (https://creativecommons.org/li- censes/by-nc-sa/4.0/) Cite this as: Sijabat, Y. Y., Setyati, W. A., & Hartati, R. (2022). Phytochemical Screening and Toxicity Test of Various Extracts from Microalga Dunaliella salina. Jurnal Ilmiah Perikanan dan Kelautan, 15(1):179-188. http://doi.org/10.20473/jipk.v15i1.36282 Copyright ©2023 Faculty of Fisheries and Marine Universitas Airlangga JIPK (JURNAL ILMIAH PERIKANAN DAN KELAUTAN) Scientific Journal of Fisheries and Marine Short Communication JIPK. Volume 15 No 1. April 2023 e-ISSN:2528-0759; p-ISSN:2085-5842 Sinta 1 (Decree No: 158/E/KPT/2021) Available online at https://e-journal.unair.ac.id/JIPK Abstract Microalgae are single celled microorganisms as the primary producers in the water food cycle. Microalgae bioactive compounds was estimated to be 10 times more diverse than compounds produced by land plants. Microalgae use nutrients more efficiently to grow, metabolize, and produce chemical compounds. Dunaliella salina is a species of chlorophyte microalgae with a lot of potential to be used in various fields. This study aimed to determine the phytochemical compound content and the value of lethal toxicity (24-hour LC50) in microalgae D. salina extract with different solvents. The multistage maceration method uses n-hexane, ethyl acetate, and methanol to extract samples. Phytochemical screening uses reagents according to the content of secondary metabolites. The Brine Shrimp Lethality Test method is used to test toxicity. The extracts were tested by using 10 Artemia salina against five concentrations, namely 0, 1, 10, 100, and 1000 ppm. Toxicity data were processed through probit analysis to get the 24-hour LC50 value. The results showed that alkaloids, steroids, triterpenoids, and phenols were found in the methanol, ethyl acetate, and n-hexane extracts. Saponin were found in the methanolic extracts. Flavonoid were found in the methanol and ethyl acetate extracts. The 24-hour LC50 value of the n-hexane extract was 276 ppm, the methanol extract was 811 ppm, and ethyl acetate extract was 673 ppm. The three extracts were included in toxic category. Extracts of microalgae D. salina have plenty secondary metabolite, that can be used in various fields and holds the potential as an anticancer.