International Journal of Scientific Research in Engineering and Management (IJSREM) Volume: 08 Issue: 04 | April - 2024 SJIF Rating: 8.448 ISSN: 2582-3930 © 2024, IJSREM | www.ijsrem.com DOI: 10.55041/IJSREM30988 | Page 1 Comparative studies on enzymatic efficacy and microbiological properties of Garbage Enzyme produced from various fruits and vegetables peels in biodegradation of cow dung Swati Rose Toppo Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee Vishwavidyalaya Bilaspur Chhattisgarh - 495 009, INDIA E-mail: swatirosetoppo@bilaspuruniversity.ac.in ORCID ID -0000-0002-1192-4247 ABSTRACT The present work was conducted to study the enzymatic potentials and microbiological properties of Garbage Enzyme produced from various fruits and vegetables peels. Completely randomized designed experiment was framed with seven treatments each with fruits and vegetable peels separately. All the treatments exhibited positive result for Amylase Test, Lipase Test, Cellulase Test, Urease Test, Protease Test, MR Test and Alcohol and low pH, indicating that Garbage enzyme produced from fruit and vegetable wastes contained mixture of enzymes, alcohol and acid brought about by microbial fermentation. Highest enzymatic efficacy was shown by T3- Pomegranate followed by T6- Banana. GE produced by vegetable wastes was less effective than fruit peels and maximum enzymatic activity exhibited by T1-Radish. A total of 27 amylase producing bacteria, 27 protease producing bacteria, 26 lipase producing bacteria, 26 cellulase producing bacteria 24 urease producing bacteria, 21 pectinase producing bacteria and 01 Amylase producing fungus was screened isolated from all the treatments. Garbage enzyme treatments in tray assay neutralized the alkaline pH of cow dung as garbage enzyme is acidic in nature. Highest Nitrogen (172 Kg/Hec) content was found when treated with garbage enzyme produced with banana peel. It is concluded that Garbage enzyme contains various enzymes, acid, and alcohol, can be utilized as a low cost alternative to biodegrade wastes to reduce pollution load of our environment. Keywords: Garbage Enzyme, vegetable and fruit wastes, enzyme activity, biodegradation INTRODUCTION Dr. Rosukon Poompanvong, the founder of the Thai Organic Farming Association, conducted the initial study on eco-enzyme during the 1980s. Researcher in Naturopathy from Penang, Malaysia, Dr. Joean Oon, popularised the use of eco-enzymes. According to the Nusantara Eco Enzyme Community (KEEN) socialisation materials, 70% of the waste that is disposed of in Final Disposal Sites (TPA) is organic waste. This waste reduces the amount of plastic recycling, releases odours into the environment, and increases the risk of explosions and organic waste decomposition. generate gaseous methane. Given that 60% of Indonesia's trash is produced for home use, it is imperative to reprocess organic waste into eco-enzymes. This information comes from the Sustainable trash Indonesia research. (Setyaka, 2020; Adelliya, 2021). The term "garbage enzyme" (GE) refers to "kitchen waste ferments," an organic substance made from fruit and vegetable peels and sugar (molasses, brown sugar, or jaggery) simply fermented in water. Garbage enzymes have been reported to have use as a cleanser, deodorizer, insecticide, soil conditioner, and insect repellent (Sarabhai and Arya, 2019). Garbage enzyme can be utilized as a low-cost alternative to improve wastewater treatment processes. Garbage enzyme may be used effectively in the treatment of greywater (Nazim and Meera, 2013). Liquid manure was prepared by fermenting the mixture of kitchen wastes in our own way i.e. potato peel (500g), carrot