© 2023 The Author(s). This article is open access Jurnal Biologi Tropis This article is licensed under a Creative Commons Attribution 4.0 International License. Original Research Paper Effectiveness of Plant Bioactive Compounds as Colorectal Cancer Cell Line Inhibitors: A Systematic Review Fadillah 1 , Aldi Tamara Rahman 1 , Aulia Rahmi 1 , Nurul Annisa 1 , Muhammad Hamdi Ibrahim 1 , Rihadatul Aisyah 1 , Reziq Marchellino Irwan 1 , Rita Maliza 1* 1 Biology Departement, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, Indonesia; Article History Received : October 02 th , 2023 Revised : October 29 th , 2023 Accepted : November 13 th , 2023 *Corresponding Author: Rita Maliza, Biology Departement, Faculty of Mathematics and Natural Science, Andalas University, Limau Manis, Padang, Indonesia Email: ritamaliza@sci.unand.ac.id Abstract: Cancer is a non-communicable disease and one of the highest causes of human death. It has been well documented that active substances have been shown in vitro to have anticancer properties. The purpose of this article is to examine the therapeutic implications of active substances involved in colorectal anticancer activity. We looked through the databases of PubMed, Science Direct, and Google Scholar in our systematic review to find papers released between 2013 and 2023. Ten studies were selected and published between 2013 and 2023. From the results obtained on plant data used as colorectal anticancer, it was reported that plants contain active phenolic compounds, flavonoids, alkaloids, gallic acid, saponins, triterpenoid/steroid, hydroquinones, triterpenoids, phenols, glycosides, quercetin, kaempferol, artemisinin, and tannins that can act as colorectal anticancer agents and are proven to be an inhibitor of cell line growth in colorectal cancer. Keywords: Anti-cancer, cell culture, colorectal. Introduction Colorectal cancer is one of the most common malignant tumors in the world, and in recent years the morbidity and mortality rates have continued to increase due to unbalanced diets such as consuming foods that are high in fat and low in fiber (Cai & Zhigang, 2021). Consuming high-fat foods will lead to increased levels of cholesterol, triglycerides, unsaturated fatty acids, and bile acids. These cholesterol and bile acids will be converted into secondary bile acids, cholesterol metabolites, and toxic substances by bacteria in the colon that can damage the colon mucosa, triggering inflammation (Harahap, 2019). Inflammation in this mucosa, resulting some disorders such as colitis and IBD (inflammatory bowel disease) that can increasing risk of colorectal cancer (Bajpai et al, 2019). Tertiary hospital-based survey in Indonesia revealed that CRC accounted for 73.7% of all gastrointestinal malignancies in 2002-2011(Makmun, 2014). In Indonesia, colorectal cancer ranks fourth with an incidence rate of 12.1 per 100,000 population and a mortality rate of 6.9 per 100,000 population (Ferlay et al., 2021). Cell culture has marked a major change in research, one of them is research on cancer cells. Cell culture is one of methods to test the cytotoxicity of potential bioactive compounds against cancer cell lines. (Rodríguez-Hernández et al., 2014). In the medical world, cells derived from certain tissues or organs can be used to research or diagnose a disease, for example, in viral or bacterial infections and in vitro studies for cancer (Thorpe, 2007). Animal cell culture involves the placement of one or more living cells of animal origin in an isolated environment whose physicochemical properties are able to mimic their physiological conditions (Rodríguez-Hernández et al., 2014). Based on the source of cells used, cells can be divided into two categories: primary cells and cell lines. Primary cultures facilitate the growth of