129 ECTI TRANSACTIONS ON COMPUTER AND INFORMATION TECHNOLOGY VOL.13, NO.2 November 2019 Identification of L-Theanine Acid Effectiveness in Oolong Tea on Human Brain Memorization and Meditation Wanus Srimaharaj 1 , Supansa Chaising 2 , Punnarumol Temdee 3 , Phakkharawat Sittiprapaporn 4 , and Roungsan Chaisricharoen 5 ABSTRACT Oolong tea has L-theanine acid, which have an impact on human brain wave activity, especially for memorization and meditation. To describe the change in the mentioned states, it requires investi- gating theta and alpha waves. Higher attention fre- quency of brain waves indicates an improvement men- tal activity. Therefore, this study proposes a method to identify the effect level of L-theanine acid in Oo- long tea focused on changing memorization and medi- tation states individually. It performs the detection of electroencephalography (EEG), statistical analysis, and data classification with specific factors to digest the distinct effect levels. The human brain waves are detected via Neurosky Mindwave Mobile during the book reading state, comparing before drinking and after drinking conditions. The detected signals are converted into voltage values and analyzed a paired sample t-test for defining the difference between the two states statistically. Moreover, an artificial neu- ral network is used to classify the effect level with associated factors including gender, age, and body mass index (BMI) into low, medium, and high lev- els. It provides 90% accuracy of 17 participants with low, 13 participants with medium, and 6 participants with high levels. The experimental results show that the level of both memorization and meditation is sta- tistically significantly increased after drinking. The instances are presented as scattered data separately. Although L-theanine in Oolong tea increases the men- tal state, it does not involve the specific factors in this study. Hence, it has different effects on a particular person. Keywords: Human brain, Memorization, Medita- tion, Oolong Tea, L-theanine, Neural Network, Elec- troencephalography Manuscript received on June 30, 2018 ; revised on July 29, 2019. Final manuscript received on January 15, 2020. 1,2,3,5 The authors are with School of Information Technology, Mae Fah Luang University, Chiang Rai, Thailand., E-mail: wanus.sri10@lamduan.mfu.ac.th, su- pansa.cha10@lamduan.mfu.ac.th, punnarumol@mfu.ac.th and roungsan.cha@mfu.ac.th 4 The author is with School of Anti-Aging and Regenerative Medicine, Mae Fah Luang University, Bangkok, Thailand., E- mail: wichian.sit@mfu.ac.th 1. INTRODUCTION Besides water, tea is the cheapest beverage for con- sumers. Nevertheless, the low price is not the only reason that raises the amount of tea consumption. Shreds of evidence support that tea has many health benefits [1]. It is the best reason why tea is widely popular in the common health markets. Previously, the investigation of tea product exportation in China, the largest tea-producing country stated that green tea is about 50%, black tea is about 30%, and oth- ers are 20% of the total exported tea [2]. Naturally, tea has famous properties to dissolve the fat in the arteries and slow aging by having substances working against free radicals. A tea leaf contains lots of nu- trients, which benefit a human health, especially by providing body refreshment. There are amino acids, e.g., catechin polyphenols, caffeine, protein, potas- sium, some vitamin types, and plenty of other nutri- ents inside. L-Theanine is one of the predominant amino acids found in typical tea leaves, which directly affects the mental state [3]. It can help to block the binding of L- glutamic acid to the glutamate receptor in the brain. L-theanine in traditional tea is proven to have their beneficial effects regarding moods [4]. In addition to other popular tea types, Oolong tea also has sufficient L-theanine to relax the human brain. It has been tested with lab mice by Osaka Institute for Health Care Science in Japan [5]. The results indicated that the stress of experimental mice had been lowered up to 18% due to the natural polyphenols is the main stress reducer. Since tea is one of the most popular beverages, there have been many types of research about L- theanine acid and human brain interaction. The previous study performed electroencephalographic (EEG) to demonstrate the effect of L-theanine acid on human brain waves [6]. Increasing alpha wave activ- ity is related to the state of relaxation or meditation in the human central nervous system [7]. Consum- ing L-theanine and caffeine can also improve inter- sensory attention, especially learning ability related to theta wave for improvement of memorization [8]. Normally, the central nervous system can be alert and relaxed at the same time. However, a condition of relaxation from the effect of L-theanine does not in-