CHEMICA: Jurnal Teknik Kimia ISSN 2355-8776 Vol. 10, No. 3, Dec 2023, pp. 144-150 144 http://dx.doi.org/10.26555/chemica.v10i3.27898 http://journal.uad.ac.id/index.php/CHEMICA/ chemica@che.uad.ac.id Evaluation and Comparison Anti-aging Facial Serum from Algae Extract Adi Permadi a,1,* , Abdul Aziz b,2 , Noorpani Ramadani a,3 , Maryudi a,4 , Totok Eka Suharto a,5 , Sami Nazzal b,6 a Department of Chemical Engineering, Faculty of Industrial Technology, Universitas Ahmad Dahlan, Yogyakarta, Indonesia b Department of Pharmaceutical Sciences, School of Pharmacy, Health Sciences Center Texas Tech University, Dallas, United States of America 1 adi.permadi@che.uad.ac.id *; 2 abdul2000020038@webmail.uad.ac.id; 3 noorpani2000020016@webmail.uad.ac.id; 4 maryudi@che.uad.ac.id; 5 totok.suharto@che.uad.ac.id; 6 sami.nazzal@ttuhsc.edu * corresponding author 1. Introduction Interest in cosmetics now no longer looks at gender and class. This is due to the development of the cosmetics industry, which is no longer segmented and targets all age ranges, young and old, even children, and no longer looks at gender [1]. However, along with the increasing number of cosmetics consumption in Indonesia, various cosmetic products have emerged that provide instant results but ignore the health aspects of the user [2]. Natural ingredients for medical and cosmetic purposes are increasingly being used in developed and developing countries [3]. This is due to increasing public literacy regarding natural ingredients, where natural ingredients will minimize side effects. The natural ingredients in question can be obtained from microalgae [4]. Microalgae can produce primary compounds such as protein, carbohydrates, and fat. Microalgae also contain active components that can be used in industrial fields such as food, pharmaceuticals, nutraceuticals, and cosmetics [5]. Microalgae contain active components such as antimicrobial, antifungal, and antiviral activities, as well as antioxidants and antibacterial substances [6]. Chlorella and spirulina are natural antioxidants that have the potential to delay, slow down, and prevent the oxidation process [7]. ARTICLE INFO ABSTRACT Article history Received November 12, 2023 Revised December 16, 2024 Accepted December 17, 2024 The demand for cosmetics in Indonesia is relatively high, so cosmetics can now be considered a primary need. Therefore, various cosmetic products have emerged that are cheap and provide instant results but ignore the health aspects of the user. Several cosmetic products make a breakthrough by using natural ingredients. One natural material that can be used is microalgae, which can produce bioactive compounds and has a relatively faster production process than other natural materials. In this research, the microalgae used were Chlorella sp., Spirulina sp., and Sargassum sp. This research aims to make a serum based on the Chlorella sp., Spirulina sp., and Sargassum sp. microalgae. The research results showed that the standard testing of simplicial facial serum extracts of Spirulina sp., Chlorella sp., and Sargassum was by SNI No. 16-4399-1996. All the metrics analyzed, such as organoleptic characteristics, pH, specific gravity, viscosity, active compounds, and microbiological contamination, have successfully fulfilled the required requirements in SNI No. 16-4399-1996. The results of antioxidant activity testing showed that Chlorella sp. had higher antioxidant activity than the other three types of samples. However, the antioxidant results obtained are still meager and relatively weak, which means this serum does not fully contribute to antiaging. Further research needs to be carried out to obtain serum from microalgae with high levels of antioxidants, including using fresh simplicia, elevating algae concentration, or optimizing the operating conditions. This is an open access article under the CC–BY-SA license. Keywords Antioxidant Chlorella Sargassum Serum Spirulina