~ 702 ~ International Journal of Chemical Studies 2017; 5(6): 702-706 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2017; 5(6): 702-706 © 2017 IJCS Received: 22-09-2017 Accepted: 25-10-2017 R Kavitha Department of Chemistry, Kunthavai Naacchiyaar Government Arts College for Women (Autonomous) Thanjavur, Tamil Nadu, India AM Uduman Mohideen P.G. and Research Department of Chemistry, Khadir Mohideen College, Adirampattinam, Thiruvarur District, Tamil Nadu, India Correspondence R Kavitha Department of Chemistry, Kunthavai Naacchiyaar Government Arts College for Women (Autonomous) Thanjavur, Tamil Nadu, India Exploration of phytocompounds in Abelmoschus moschatus flowers using HPLC, Uv-Vis and FTIR techniques R Kavitha and AM Uduman Mohideen Abstract Medicinal plants are assuming greater importance in the primary health care of individuals and communities in many developing countries. The bioactive components of Abelmoschus moschatus flowers have been evaluated using HPLC, UV VIS and FTIR. The phytochemical screening of Abelmoschus moschatus flowers showed that the presence of flavonoids, terpenoids, tannin, saponins, glycosides, triterpenoids, phenol, anthroquinones were present in aqueous, ethanol and ethyl acetate extracts. The UV- VIS profile showed the occurrence of peaks at 207-380 nm reveals the presents of flavonoids in the Abelmoschus moschatus. The results of FTIR analysis confirmed the presence of phenol, alkanes, Alkenes, alcohol, aromatic, Aliphatic amines and amine compound. The results of this study offer a platform of using Abelmoschus moschatus flower as herbal alternative for various diseases including diabetic, cardiovascular etc. The results of this study offer a platform of using Abelmoschus moschatus flowers as herbal alternative for various diseases including cancer and diabetic. Keywords: GC MS, HPLC, UV-VIS, FTIR, Abelmoschus moschatus 1. Introduction Medicinal plants are believed to be much safer and proved elixir in the treatment of various ailments. They are assuming greater importance in the primary health care of individuals and communities in many developing countries. There has been an increase in demand in international trade because of very effective, cheaply available, supposedly have no side effects and used as an alternative to allopathic medicines [1] . Phytochemicals simply means plant chemicals. “Phyto” is the Greek word for plant. Phytochemicals are classified as primary or secondary constituents, depending on their role in plant metabolism. Secondary metabolism in a plant plays a major role in the survival of the plant in its environment. In addition, these compounds may be responsible for the beneficial effects of fruits and vegetables on an array of health related measures [2] . Chemical principles from natural sources have become much simpler and have contributed significantly to the development of new drugs from medicinal plants [3] . The valuable medicinal properties of different plants are due to presence of several constituents i.e. saponins, tannins, alkaloids, alkenyl phenols, glycol-alkaloids, flavonoids, sesquiterpenes lactones, terpenoids and phorbol esters [4] . Among them some are act as synergistic and enhance the bioactivity of other compounds. Within a decade, there were a number of dramatic advances in analytical techniques including HPLC, UV, FTIR, NMR and GC-MS that were powerful tools for separation, identification and structure determination of phytochemicals (Roberts and Xia, 1995) [5] . The aim of this study is to determine the bioactive compounds present in the Abelmoschus moschatus flower extract with the aid of HPLC, UV-VIS and FTIR Techniques, which may provide an insight in its use of tradition medicine. 2. Materials and Methods Plant materials The flowers of Abelmoschas moschatus were collected from Thanjavur, Tamil Nadu, India from a single tree. Preparation of Extracts The Abelmoschas moschatus flowers were first washed well and dust was removed from the flower.