Effects of platelet rich fibrin alone used with rigid titanium barrier Hakan Ozdemir a , Seref Ezirganli b , M. Isa Kara c, *, Ahmet Mihmanli b , Emre Baris d a Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey b Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey c Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Izmir Katip Celebi University, Izmir, Turkey d Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey 1. Introduction The etiologies of alveolar bone defects include advanced periodontal disease, respective surgery, especially resulting from trauma or tumor surgery, large-bone defects. 2 To overcome these problems, guided bone regeneration (GBR) is used with numerous grafting materials including autografts, allografts, xenografts and alloplastic grafts. Among grafting materials, autogenous bone grafts possess superior osteogenic and osteoinductive properties, they are considered the gold standard for grafting materials. 3 However, a r c h i v e s o f o r a l b i o l o g y x x x ( 2 0 1 2 ) x x x – x x x * Corresponding author at: Izmir Katip Celebi University, Faculty of Dentistry, 35180 Izmir, Turkey. Tel.: +90 232 3293535; fax: +90 232 3293999. E-mail address: phismer@yahoo.com (M. Isa Kara). Abbreviations: BCP, biphasic calcium phosphate; bTCP, beta-tricalcium phosphate; ABB, anorganic bovine bone; GBR, guided bone regeneration; HA, hydroxyapatite; PRF, platelet rich fibrin; PRP, platelet rich plasma. a r t i c l e i n f o Article history: Accepted 28 September 2012 Keywords: Guided bone regeneration Platelet rich fibrin Anorganic bovine bone Biphasic calcium phosphate Titanium barrier Rabbit a b s t r a c t Objective: Platelet-rich fibrin (PRF) is a platelet and leukocyte rich and platelet preparation that concentrates various growth factors and therefore has the potential to be used as regenerative treatment. The aim of study was to assess the effects of platelet rich fibrin (PRF) on bone augmentation when used in conjunction with titanium barrier a rabbit calvaria model. Study design: Twenty-four adult male New Zealand rabbits were used in this study. Two titanium barriers were fixed on each rabbit’s calvarium. The rabbits were divided into four groups (group one is control and the other three groups are experimental) and each group contains 6 animals. PRF, anorganic bovine bone (ABB), and biphasic calcium phosphate (BCP) were used with titanium barriers in the experimental groups. Any materials were not used in the control group. Half of the animals were sacrificed after 1 month, and the rest were sacrificed after 3 months. Histomorphometric evaluation was carried out in order to compare new bone formation among the groups. Results: Significantly more new bone area was noted in the PRF alone group than in the control group, no statistically significant differences were found among PRF, BCP and ABB groups after 1 month. PRF and ABB also had superior effects in new bone formation area control to the BCP group after 3 months. Conclusion: PRF may offer the ease of use, simple handling, and enhanced delivery of growth factors during the bone augmentation procedures. When used in conjunction with the titanium barriers, PRF use can increase the quality of the newly formed bone and enhance the rate of bone formation due to the concentration of growth factors. # 2012 Elsevier Ltd. All rights reserved. AOB-2893; No. of Pages 8 Please cite this article in press as: Ozdemir H, et al. Effects of platelet rich fibrin alone used with rigid titanium barrier. Archives of Oral Biology (2012), http://dx.doi.org/10.1016/j.archoralbio.2012.09.018 Available online at www.sciencedirect.com journal homepage: http://www.elsevier.com/locate/aob 0003–9969/$ – see front matter # 2012 Elsevier Ltd. All rights reserved. http://dx.doi.org/10.1016/j.archoralbio.2012.09.018