_____________________________________________________________________________________________________ *Corresponding author: E-mail: dominicreuben@yahoo.com; British Microbiology Research Journal 16(3): 1-12, 2016, Article no.BMRJ.28294 ISSN: 2231-0886, NLM ID: 101608140 SCIENCEDOMAIN international www.sciencedomain.org Microorganisms Associated with Biogas Production Using Vegetable (Telfairia occidentalis) Wastes, Banana Peel and Pig Dung as Substrates B. E. Asikong 1 , S. O. Idire 1 and D. R. Tiku 1* 1 Department of Microbiology, University of Calabar, Calabar, Nigeria. Authors’ contributions This work was carried out in collaboration between all authors. Author BEA designed the study, performed the statistical analysis, wrote the protocol, wrote the first draft of the manuscript and managed literature searches. Authors SOI and DRT managed the analyses of the study and literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/BMRJ/2016/28294 Editor(s): (1) Laleh Naraghi, Plant Disease Research Department, Iranian Research Institute of Plant Protection, Tehran, Iran. Reviewers: (1) Muhammad Imran, Institute of Biochemistry and Biotechnology, University of Veterinary and Animal sciences, Lahore, Pakistan. (2) Yusufu Risasi Rajabu, University of Dodoma, Tanzania. Complete Peer review History: http://www.sciencedomain.org/review-history/15703 Received 13 th July 2016 Accepted 31 st July 2016 Published 7 th August 2016 ABSTRACT The research study was aimed at investigating microorganisms associated with biogas production using vegetable (Telfairia occidentalis) wastes, banana peel, and pig-dung as substrates. Marian market, Watt market and University of Calabar pig farm were randomly sampled within Calabar metropolis for collection of samples. The study was completed within a period of six month. Standard microbiological methods and anaerobic biodigesters were used to screen the isolates and the wastes substrate for biogas production. Analysis revealed that the temperature of raw substrates ranged between 21°C and 39°C while the pH varied bet ween 6.10 and 7.21 during digestion. Highest mean bacterial counts was 8.87±3x10 6 cfu/g and fungal count of 5.67±10 5 cfu/g were obtained in the combined substrates of banana peel, vegetable waste and pig dung (BP + VW + PD) before digestion, as compared to mean bacterial counts of 8.62±1.4x10 6 fu/g and fungal counts of 5.55±1.7x10 5 cf/g obtained during digestion. Anaerobic bacteria isolated were identified as, Pseudomonas sp, Escherichia coli, Bacillus sp, Salmonella sp, Staphylococcus aureus, Serratia sp, Shigella sp, Micrococcus sp, Proteus vulgans, Citrobacter sp and Klebsiella sp, while fungi isolated Original Research Article