Setting: Gynecologic oncology referral center. Patients: Consecutive patients with early stage endometrioid endometrial cancer scheduled for surgical treatment: total laparoscopic hysterectomy, bilateral salpingo-oophorectomy, SLNs mapping. Intervention: Mapping technique consisted in a intraoperative hysteroscopic peritumoral injection of 5mg of ICG (after tubal coagulation) followed by L-NIR Fluorescence mapping using the Storz Professional Image Enhancement System (SPIES) with D-Light P light source (Karl Storz Endoskope GmbH & Co. KG, Tuttlingen, Germany). Evaluation of SLNs DR was performed, as well as site of mapping. Measurements and Main Results: A total of 53 procedures were performed. Mean age was 60 years (28-80) and mean BMI was 28.2 Kg/ m2(19-43). At least one SLN was detected in the 88.7% of the whole population (47/53). When evaluating the latter 37 patients in which mapping was performed with a new scope allowing dissection under NIR vision, the DR was 94.6% (35/37). Mean number of detected SLNs was 4.2 (1-8) and in more than half of cases the aortic area mapped. Bilateral pelvic mapping was found in the 74.5% of cases (35/47). Three patients had SLNs involvement: one in the pelvic area only, one both in the pelvic and aortic area and one presented two metastatic aortic SLNs (1 partial node metastasis and 1 node with isolated tumor cells) with negative pelvic SLNs. No adverse events were reported. Conclusion: Laparoscopic mapping following hysteroscopic injection of ICG has comparable DR to both radioactive tracers series and ICG series with cervical injection, overcoming the usage of radioactive substances. As reported in literature, hysteroscopic injection of tracer leads to a higher mapping in the aortic area compared to cervical injection. Further investigation is warranted on this topic. 236 Open Communications 21 - Oncology (2:15 PM - 3:15 PM) 3:01 PM – GROUP B The Role of Laparoscopic Radical Hysterectomy in Early-Stage Adenocarcinoma of the Uterine Cervix Park J-Y, Kim D-Y, Suh D-S, Kim J-H, Kim Y-M, Kim Y-T, Nam J-H. Department of Obstetrics and Gynecology, Unversity of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea Study Objective: To compare long-term survival outcomes and patterns of recurrence of laparoscopic radical hysterectomy (LRH) and open radical hysterectomy (ORH) in early-stage adenocarcinoma of the cervix. Design: Retrospective comparative study. Setting: University hospital in Korea. Patients: 293 patients with stage IA2-IIA cervical cancer with adenocarcinoma. Intervention: Laparoscopic radical hysterectomy and open radical hysterectomy. Measurements and Main Results: In total, 186 patients underwent LRH and 107 underwent ORH. There was no difference between the two surgery groups in clinicopathologic characteristics. There were no differences in disease-free survival (DFS) and overall survival (OS) between the LRH and ORH groups (88.7% vs. 84.1%, P = .725; and 93.0% vs. 86.9%, P = .735) in univariate analysis and in multivariate analysis after adjusting for other significant prognostic factors. There was no difference in the patterns of recurrence between the two surgery groups (P = 0.220). The median time interval between surgery and the first recurrence were 25 months (range, 3-100 months) for LRH group and 14 months (range, 3-128 months) for ORH group (P = 0.230). The LRH group showed significantly fewer postoperative complications (P \ .001), less estimated blood loss (P \ .001), faster bowel movement recovery (P \ .001), shorter postoperative hospital stay (P \ .001), and a lower rate of wound dehiscence, ileus, lymphedema, and lymphocele and pelvic abscess (P = .004, .011, .017, and .040, respectively). Conclusion: LRH has comparable survival outcomes with ORH and did not affect the pattern of recurrence in early-stage adenocarcinoma of the uterine cervix. The surgical outcomes were more favorable than ORH. 237 Open Communications 21 - Oncology (2:15 PM - 3:15 PM) 3:08 PM – GROUP B DJ-1 a New Biomarker to Preoperatively Discriminate Between High-Risk and Low-Risk Endometrial Cancer Venturella R, Di Cello A, Rania E, Marra ML, Rocca ML, Di Sanzo M, Morelli M, Zullo F, Costanzo FS. Unit of Obstetrics and Gynecology, Pugliese Ciaccio Hospital, Department of Experimental and Clinical Medicine, Magna Graecia University, Catanzaro, Italy Study Objective: To validate the accuracy of DJ-1 dosage for discriminating preoperatively between high-risk and low-risk endometrial (EC). To evaluate the potential prognostic value of this biomarker. Design: Prospective experimental study. June 2013 to December 2015. Setting: Unit of Obstetric and Gynecology, Pugliese Ciaccio Hospital, Department of experimental and clinical Medicine,UMG, Catanzaro. Patients: 101 patients affected by EC and underwent surgical treatment were prospectively enrolled as cases and 44 healthy women were enrolled as controls. Intervention: Clinical, biochemical, surgical and pathological data were collected at diagnosis and follow-up. Measurements and Main Results: The association between pathological factors (grading and hystotype) and DJ-1 levels variation were analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the role of preoperative serum DJ-1 in diagnosis of EC and in prediction of the class of risk. Survival analysis was also performed in our population divided according to the preoperative DJ-1 serum levels (DJ-1 positive and DJ-1 negative patients). Serum DJ-1 levels were significantly higher in high-risk EC compared with low-risk EC (p=0.001). The ROC curve analysis shows that DJ1 has a very good diagnostic accuracy in discriminating between EC patients and controls (AUC 0.883; CI 0.829- 0.937) and an excellent accuracy in discriminating, among EC patients, low and high-risk cases (AUC 0.991; CI 0.980-0.999). The most accurate cut- off values to discriminate between EC patients and controls, between low- and high-risk were identified. Survival analysis revealed that patients with high DJ-1 levels had a poor prognosis compared to those with low DJ-1 levels. Conclusion: Preoperative serum DJ-1 is a new accurate serum biomarker for EC diagnosis and, moreover, for high-risk EC identification. DJ-1 could be considered as an important prognostic predictor for patients with EC and it should be taken into consideration when surgical management has to be performed. 238 Open Communications 22 - Laparoscopic Surgeries (3:25 PM - 5:05 PM) 3:25 PM – GROUP A Surgical Experience with Ulipristal Acetate or Gonadotropin Releasing Hormone Agonists for Uterine Fibroids Wais M, 1 Lee S, 2 Liu G, 3 Pham A, 4 Tai M, 5 Murji A. 6 1 Department of Obstetrics and Gynaecology, University of Toronto, Toronto, Ontario, Canada; 2 North York General Hospital, Toronto, Ontario, Canada; 3 Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada; 4 St. Joseph’s Health Centre, Toronto, Ontario, Canada; 5 Toronto East General Hospital, Toronto, Ontario, Canada; 6 Mount Sinai Hospital, Toronto, Ontario, Canada Study Objective: Our objective was to evaluate if there exists a difference in surgical experience at myomectomy between fibroids that are pre-treated with Ulipristal Acetate (UA) compared to no medical pre-treatment. Design: A multi-center prospective study of hysteroscopic, open and laparoscopic myomectomy procedures was conducted using a web-based questionnaire. Based on the responses, a global surgical experience score (SES) was calculated for every procedure. Setting: Five hospitals, both academic and community, across a large Canadian city. S100 Abstracts / Journal of Minimally Invasive Gynecology 23 (2016) S1–S252