Research Article Exacerbation of Thrombotic Responses to Silver Nanoparticles in Hypertensive Mouse Model Zannatul Ferdous, 1 Sumaya Beegam, 1 Nur E. Zaaba, 1 Ozaz Elzaki, 1 Saeed Tariq, 2 Yaser E. Greish, 3 Badreldin H. Ali, 4 and Abderrahim Nemmar 1,5 1 Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE 2 Department of Anatomy, College of Medicine and Health Science, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE 3 Department of Chemistry, College of Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE 4 Department of Pharmacology and Clinical Pharmacy, Sultan Qaboos University, P.O. Box 35, Muscat 123, Al-Khod, Oman 5 Zayed Center for Health Sciences, United Arab Emirates University, UAE Correspondence should be addressed to Abderrahim Nemmar; anemmar@uaeu.ac.ae Received 31 August 2021; Accepted 30 November 2021; Published 15 January 2022 Academic Editor: Vladimir Jakovljevic Copyright © 2022 Zannatul Ferdous et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. With advent of nanotechnology, silver nanoparticles, AgNPs owing majorly to their antibacterial properties, are used widely in food industry and biomedical applications implying human exposure by various routes including inhalation. Several reports have suggested AgNPs induced pathophysiological effects in a cardiovascular system. However, cardiovascular diseases such as hypertension may interfere with AgNPs-induced response, yet majority of them are understudied. The aim of this work was to evaluate the thrombotic complications in response to polyethylene glycol- (PEG-) coated AgNPs using an experimental hypertensive (HT) mouse model. Saline (control) or PEG-AgNPs (0.5 mg/kg) were intratracheally (i.t.) instilled four times, i.e., on days 7, 14, 21, and 28 post-angiotensin II-induced HT, or vehicle (saline) infusion. On day 29, various parameters were assessed including thrombosis in pial arterioles and venules, platelet aggregation in whole blood in vitro, plasma markers of coagulation, and fibrinolysis and systemic oxidative stress. Pulmonary exposure to PEG-AgNPs in HT mice induced an aggravation of in vivo thrombosis in pial arterioles and venules compared to normotensive (NT) mice exposed to PEG-AgNPs or HT mice given saline. The prothrombin time, activated partial thromboplastin time, and platelet aggregation in vitro were exacerbated after exposure to PEG-AgNPs in HT mice compared with either NT mice exposed to nanoparticles or HT mice exposed to saline. Elevated concentrations of fibrinogen, plasminogen activator inhibitor-1, and von Willebrand factor were seen after the exposure to PEG-AgNPs in HT mice compared with either PEG-AgNPs exposed NT mice or HT mice given with saline. Likewise, the plasma levels of superoxide dismutase and nitric oxide were augmented by PEG-AgNPs in HT mice compared with either NT mice exposed to nanoparticles or HT mice exposed to saline. Collectively, these results demonstrate that PEG-AgNPs can potentially exacerbate the in vivo and in vitro procoagulatory and oxidative stress effect in HT mice and suggest that population with hypertension are at higher risk of the toxicity of PEG-AgNPs. 1. Introduction Silver nanoparticles (AgNPs) became one of the most inves- tigated engineered nanomaterials during the past few years, given the fact that these nanomaterials proved to have inter- esting, challenging, and promising characteristics suitable for various household and biomedical applications [1–3]. The widespread application in turn results in environmental contamination and human exposure raising serious concern about their potential adverse effects and toxicity on human health [2, 4]. Of all the various routes of exposure of nano- particles reported so far, pulmonary exposure provides a major a potential route to aerosolized AgNPs used in health sprays, nebulizers, deodorants, and disinfectants [5]. Hindawi Oxidative Medicine and Cellular Longevity Volume 2022, Article ID 2079630, 10 pages https://doi.org/10.1155/2022/2079630