_____________________________________________________________________________________________________ *Corresponding author: E-mail: mcgarg@amity.edu, vidushi.450@rediffmail.com; Journal of Pharmaceutical Research International 34(4B): 12-23, 2022; Article no.JPRI.79828 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Biochemical and Histological Evaluation of Penoxsulam Herbicide on an Animal Model Vidushi Chaurasia a,b* , M. L. Aggarwal b and Manoj Chandra Garg a* a Amity Institute of Environmental Sciences, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India. b Shriram Institute for Industrial Research, New Delhi, India. Authors’ contributions This work was carried out in collaboration among all authors. Author VC designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors MLA and MCG managed the analyses of the study. Author MCG managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2022/v34i4B35399 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/79828 Received 20 November 2021 Accepted 24 January 2022 Published 25 January 2022 ABSTRACT Aims: Many herbicides react to the body system and might effect the activity of hormones in the human body. The present work aims to determine the potential impact of 90 days of repeated exposure to Penoxsulam herbicide by oral gavage on the Liver, Kidney, Thyroid endocrine profile and biochemical stress in the wistar rat model. Study Design: The primary study was performed to Wistar rats grouped into 6 groups. Four groups were picked for low dose, middle dose, high dose, and high recovery dose, respectively. They were administered the Penoxsulam at dose levels of 100, 300, 500 mg/kg body weight. Similarly, 2 recovery groups were classified as control and recovery control groups, and doses were administered to them only through the corn oil (vehicle) via the oral route with the help of a suitable cannula for 90 days. Place and Duration of Study: Toxicology department, Shriram Institute for Industrial Research, Delhi (INDIA), July 2020 and June 2021. Methodology: In this study, healthy 60 male and 60 female Wistar rats aged 6-8 weeks, weighing 130-190 gm, were used. Before commencing the study, permission from IAEC (Institutional animal ethics committee) was taken for this experiment (CPCSEA). Results: This study evaluates significant changes in the body weight of rats; moreover, Original Research Article