International Journal of Research and Review Vol. 10; Issue: 5; May 2023 Website: www.ijrrjournal.com Original Research Article E-ISSN: 2349-9788; P-ISSN: 2454-2237 International Journal of Research and Review (ijrrjournal.com) 13 Volume 10; Issue: 5; May 2023 Hay Infusion is More Effective than The Yeast and Palm Sugar Combination as an Ovitrap Attractant of Aedes Aegypti Mosquitoes I Nyoman Wigraha Yoga 1 , Putu Ayu Asri Damayanti 2 , Ni Luh Putu Eka Diarthini 2 , Dewa Ayu Agus Sri Laksemi 2 1 Faculty of Medicine, Udayana University, Denpasar, Indonesia 2 Department of Parasitology, Faculty of Medicine, Udayana University, Denpasar, Indonesia Corresponding Author: I Nyoman Wigraha Yoga DOI: https://doi.org/10.52403/ijrr.20230503 ABSTRACT The Aedes aegypti mosquito is widely known as a vector for Dengue Hemorrhagic Fever (DHF). Mosquito population can be controlled by using oviposition traps (ovitrap). Attractants can be used to attract mosquitoes to lay their eggs. This study aims to compare the effectiveness of Ae. aegypti mosquitoes ovitrap attractant between hay infusion, the yeast-palm sugar combination and ovitrap standard without attractant. The research method was an experimental research posttest only control group design. The results showed that the highest number of eggs during the 4 days of observation was in the hay infusion with an average number of eggs 253,44±58,445 followed by ovitrap without attractant and palm sugar with the number of eggs 31,89±25,300 and 89,78±39,499 respectively sequentially. There was a significant difference in the mean number of eggs between 10% hay infusion and 10% yeast and palm sugar combination and water with p=0.000. The conclusion of this study is that hay infusion attractant is more effective than yeast and palm sugar combination. Keywords: Aedes aegypti, Yeast, Hay Infusion, Ovitrap, Palm Sugar. INTRODUCTION Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus which is transmitted by the Aedes aegypti’s bite. This disease is usually found in tropical regions such as Indonesia, especially after the rainy season. Based on data from the Ministry of Health of the Republic of Indonesia, in 2019 there were 13,683 confirmed cases of DHF with 132 cases of them dying in Indonesia. In 2017, there were 4499 cases of dengue fever in the province of Bali with 13 deaths. 1 There are 4 stages in mosquito life cycle, which is egg, larva, pupa, and adult mosquito. Ae. aegypti generally breeds in water reservoirs (manmade containers). 2 Larvae that have hatched will experience growth and metamorphosis into adult mosquitoes. Ae. aegypti eggs can survive for 2-3 months without being submerged in water. 3 This causes the population of Ae. aegypti increases after the rainy season. 4 Placement of mosquito eggs (oviposition) can be controlled by using ovitrap. Mosquito eggs collected in the ovitrap will be cleaned regularly to prevent the eggs from hatching. 5,6 This action will reduce the mosquito population by breaking their life cycle. Ovitrap can be used together with attractants to increase their effectiveness, several types of attractants have been studied and their effectiveness compared. Oviposition can be increased by using attractants. Attractants can be made from natural or synthetic materials. 7 The attractant that has proven to be effective is hay infusion, this attractant is easy to find