Nano-Forensics a Comprehensive Review Paikrao Hariprasad 1 *, Dipale Ashlesha 2 , Patil Anita 3 and Tajane Diksha 4 1 Assistant Professor, Department of Forensic Biology, Government Institute of Forensic Science, Nagpur, India 2 Post graduate student, Department of Forensic Biology, Government Institute of Forensic Science, Nagpur, India 3 Professor, Department of Biotechnology, Sant Gadge Baba Amravati University Amravati, India 4 Research Scholar, NAU, Navsari, India Introduction Nanotechnology is making a valuable contribution in every field. It is a widely used technique because of its ability to manipulate and characterize the matter at a level of single atoms and small atoms [1]. The word “nano” is derived from the ancient Greek “Nanos,” meaning “dwarf,” which refers to the “billionth” or a factor of 10-9. In general, to understand 1nm is about 3-10 atoms wide. It is very tiny when compared with the standard size encountered day-to-day. E.g., 1nm is 1/1000th the width of human hair. Nanotechnology described the study aspect concerning the science, engineering, and technology conducted at a scale that ranges between 1 to 100 nanometers [2]. The potential of nanotechnology in electronics, diagnostics, biosensing, imaging, optical devices, and drug delivery due to their small size, large surface area, and enhanced reactivity [3-7]. Its multi-purpose application in almost every field has made it a universal purpose technology [2]. Therefore this universal technology has a plentiful of applications in the field of forensic science. Nano-Forensic, an exquisite blend of nanotechnology and forensic science, is entirely a novel discipline in forensic science. Nano-Forensic helps identify and examine evidence at the nanoscale level as earlier, it is impossible to analyze the critical evidence because of the instrument’s detection limit. With advancements in the techniques, Nano analysis is transforming the investigation process by making them more accurate, faster, and more sensitive [8,9]. Nano forensic and other technologies have significant fingerprint analysis applications, explosive detection, drug screening, toxic substance analysis, and DNA analysis [10,11]. This review would briefly update how nanotechnology is widely used in forensic analysis of evidence. Nanomaterials for latent fingerprint analysis Nanoparticles have been used for decades to develop the latent fingerprint, with silver nanoparticles being used from 1970. The small size of nanoparticles allows them to bind with the minute ridges of a fingerprint, further enhancing the ridge details on fingerprints and Crimson Publishers Wings to the Research Mini Review *Corresponding author: Paikrao Hariprasad, Assistant Professor, Depart- ment of Forensic Biology, Government Institute of Forensic Science, Nagpur, India Submission: January 05, 2021 Published: February 03, 2021 Volume 5 - Issue 3 How to cite this article: Paikrao Hariprasad, Dipale Ashlesha, Patil Anita, Tajane Diksha. Nano-Forensics a Comprehensive Review. Forensic Sci Add Res. 5(3). FSAR. 000618. 2021. DOI: 10.31031/FSAR.2021.05.000618 Copyright@ Paikrao Hariprasad, This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use and redistribution provided that the original author and source are credited. ISSN: 2578-0042 411 Forensic Science & Addiction Research Abstract Nanotechnology is becoming popular in today’s world, with the nano-revolution touching almost in every field. It offers a promising application in electronics, diagnosis, biosensing, drug delivery, and imaging. Nano-Forensic is emerging as a novel discipline in the field of forensic science. Nanotechnology has been utilized extensively in forensic science to detect and analyse evidence at a nanoscale level. Nano analysis is transforming the investigation process by making them more accurate, faster, and more sensitive. As nanomaterials have the property of enhancing the detection limit at nanoscale level it has been widely used to detect fingerprint, explosives, unlawful drugs, toxic substances and DNA samples. Keywords: Nano-sensors; Forensic science; Nano-forensics; Nano-PCR