Advances in Pharmacology and Pharmacy 12(4): 424-429, 2024 http://www.hrpub.org DOI: 10.13189/app.2024.120414 The Effect of Oxytetracycline Administration on Tissue Disposition of Diminazene Aceturate in Apparently Healthy Sahel Goats Sarah Malgwi Gana 1 , Bukar Umaru 1 , Hyellavala Joseph Fomnya 1,* , Patrick Azubuike Onyeyili 2 1 Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria 2 Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Federal University of Agriculture Makurdi, Nigeria Received December 11, 2023; Revised March 5, 2024; Accepted May 20, 2024 Cite This Paper in the Following Citation Styles (a): [1] Sarah Malgwi Gana, Bukar Umaru, Hyellavala Joseph Fomnya, Patrick Azubuike Onyeyili, "The Effect of Oxytetracycline Administration on Tissue Disposition of Diminazene Aceturate in Apparently Healthy Sahel Goats," Advances in Pharmacology and Pharmacy, Vol. 12, No. 4, pp. 424 - 429, 2024. DOI: 10.13189/app.2024.120414. (b): Sarah Malgwi Gana, Bukar Umaru, Hyellavala Joseph Fomnya, Patrick Azubuike Onyeyili (2024). The Effect of Oxytetracycline Administration on Tissue Disposition of Diminazene Aceturate in Apparently Healthy Sahel Goats. Advances in Pharmacology and Pharmacy, 12(4), 424 - 429. DOI: 10.13189/app.2024.120414. Copyright©2024 by authors, all rights reserved. Authors agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Abstract This study aims to determine the effect of oxytetracycline administration on tissue disposition of diminazene aceturate in apparently healthy Sahel goats. Eighteen animals were used for the experiment. Sixteen of the animals were grouped into two with eight animals in each group. The first group was given only diminazene aceturate at 3.5 mg/kg intramuscularly, and the second group received diminazene aceturate (3.5 mg/kg) in combination with 20% oxytetracycline (20 mg/kg) intramuscularly. Diminazene aceturate was administered 30 minutes after the administration of 20% oxytetracycline. Two goats were sacrificed from each group at 5, 10, 15, and 20 days post-administration of the drugs.The two untreated goats were sacrificed to prepare the control tissues and standards. The concentration of diminazene aceturate in various tissues was determined using a chemical assay. Diminazene aceturate administered alone or in combination with oxytetracycline was absorbed and detected in various organs of the treated Sahel goats, with the liver and the kidney recording the highest concentrations. Tissues harvested from goats that received the drug combination presented higher concentrations than the goats treated with diminazene aceturate alone. Drug residues were detected twenty days after drug administration in most tissues of the experimental goats. Diminazene aceturate was found to be present in the tissues of goats for more than 20 days after treatment, hence, 20% oxytetracycline was found to alter the excretion style of diminazene aceturate in goats treated with 20% oxytetracycline. Keywords Diminazene Aceturate, Diminazene Assay, 20% Oxytetracycline, Sahel Goats, tissueKinetics 1. Introduction Diminazene Aceturate (DA) has been a preferred medication in the management of tsetse fly disease popularly known as trypanosomiasis or piroplasmosis which is commonly called babesiosis for over 70 years. It is mostly used in livestock production because of its high therapeutic index [1]. Diminazene Aceturate exerts its antibabesial effects through the interference of both aerobic glycolysis and DNA synthesis [2,3]. Diminazene Aceturate also binds to trypanosomal kinetoplast DNA in a non- intercalative way to achieve therapy [4]. Odika et al., [5] conducted research that shows a significantly high residue of diminazene aceturate in various tissues of Wistar rats harboring the protozoan parasite Trypanosoma brucei brucei in contrast with the rats that are not harboring the protozoan parasite Trypanosoma brucei brucei. Simultaneous administration of diminazene aceturate and