Iran. J. Chem. Chem. Eng. Research Article Vol. 38, No. 2, 2019 Research Article 9 Synthesis and Characterization of Nanoparticles Propolis Using Beeswax Shaltouki, Parisa Department of Chemical Engineering, Qhochan Branch, Islamic Azad University, Qhochan, I.R. IRAN Mohamadi, Elaheh; Moghaddasi, Mohammadali, Farahbakhsh, Afshin Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, I.R. IRAN Bahmanpour, Hooman* + Department of Environment Engineering, Shahrood Branch, Islamic Azad University, Shahrood, I.R. IRAN ABSTRACT: In order to protection, convenient release, increase of antibacterial of capsules to treatment of diseases, propolis nanoparticles encapsulate. Beeswax is used for covering because of its special physical and chemical properties, ineffective and inactivity and ease of mixing with materials without any adverse reaction. In this study, nanotechnology and renewable natural compounds of beeswax were used in the process of encapsulating for protection against adverse environmental conditions. At first, propolis nanoparticles were mixed with chloroform then ammonia buffer and Tween -80 was added to it while stirring with speed rpm 300. The mixture was shocked to form the capsule. After filtration and washing produced capsules were dried for 48 hours at room temperature. Assessment of formation and performance of the capsules was done by changing parameters such as pH, time and temperature, the loading of nanoparticles by spectrophotometry method and increasing the antimicrobial properties using microbial culture. Also, FT-IR analysis was done to prove physical transplant of wax and propolis. According to TEM images, the size of produced capsules was estimated in the range of 200 to 500 nm with 95% distribution percentages. Based on Taguchi testing, the optimum time, temperature and pH for release of encapsulated nanoparticles were 10 minutes, 43ºC and 10, respectively. KEYWORDS: Encapsulating; Propolis Nanoparticles; Capsule; Beeswax; Spectrophotometry. INTRODUCTION Beeswax is a natural biological polymer [1] containing a mixture of several non-toxic and cheap substances (esters of fatty acids, alcohols, acids, etc.). The number of reported individual components have been contained beeswax exceeds 300 which are from various species of honeybees. Depending on the honeybee species and the geographical zone, the concentrations of individual components and substance classes may have only small differences [2]. In addition, from the point of view of chemistry, it is a stable and water-repellent substance [3]. Beeswax is a highly crystalline natural product that is used in pharmaceutical, cosmetics, food and other * To whom correspondence should be addressed. + E-mail: h.bahmanpour@srbiau.ac.ir 1021-9986/2019/2/9-19 11/$/6.01