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Clinics in Oncology
2017 | Volume 2 | Article 1369 1
Keywords
Transitional pre-B ALL; Philadelphia chromosome; Cytogenetic abnormalities
Letter to Editor
A 55 year old woman was admitted to the emergency room with progressive fatigue and fever.
Her physical examination revealed fever (38.8
°
C), pallor, ecchimoses in the lower extremities.
Abnormal laboratory values included: hemoglobin: 7.7gr/dl, hematocrit: 23%, white blood cell:
33 600/µl, platelets: 20 400/µl, lactic dehydrogenase: 2632 IU. Lymphoblasts were found in the
peripheral blood smear and bone marrow aspiration revealed a hypercelluler bone marrow with
diffuse blast cell infiltration. Flow cytometric analysis was positive for TdT, CD 10, CD19, CD10,
D19, CD20, CD22, CD24, CD34, cyCD79a, HLADR and surface IgM (mu) with negative kappa and
lambda. Cytogenetic analysis of the bone marrow performed with GTG-banding with short term
unstimulated culture showed a karyotype; 46,XX,t(9,22)(q34;q11) [2]/40-44,XX[2]/46,XX [1]. Bcr-
abl was positive by RT-PCR. She was diagnosed with Philadelphia chromosome positive transitional
pre-B acute lymphoblastic leukemia (ALL) and Hyper CVAD (hyperfractionated cyclophosphamide,
vincristin, adriamycine and dexamethasone) chemotherapy and tyrosine kinase inhibitor (imatinib)
was commenced. Patient succumbed to sepsis seven days aſter starting chemotherapy.
Transitional pre-B ALL (TPB-ALL) is a subtype of ALL where lymphoblasts are between
pre-B and B stage of differentiation and is reported to consists a very small subset of pre-B ALL
patients. While Koehler et al. reported seventeen cases in a series of 1726 pediatric patients
which consists less than 2% of the cases, López-Karpovitch et al identified 4 cases of TPB-ALL
in a cohort of 60 consecutive cases of de novo adult acute ALL suggesting a higher frequency in
adults [1,2]. Transitional pre-B ALL blasts express CD10, TdT and IgM (mu) heavy chain without
kappa and lambda light chains and lacks FAB L3 morphology and the (8;14), (8;22), and (2;8)
translocations [1,2,3]. Koehler et al. [1] also showed some chromosomes abnormalities in 7 of 17
patients [1]. However no particular cytogenetic abnormality has been found to be associated with
transitional ALL. On the other hand Callera et al. [3] reported a case with ringed chromosome 16
[3]. e presented patient, to best of our knowledge is the first case of TPB-ALL with philadelphia
chromosome. Philadelphia chromosome is the most frequent cytogenetic abnormality in adult ALL,
seen in 25-30% of the cases and even more common above the age of 50 [4]. e present data is
immature to comment on the prognosis of TPB-ALL although an excellent prognosis is reported
in pediatric cases. Philadelphia chromosome remains to have a dismal prognosis even in the era of
tyrosine kinase inhibitors [5] and we believe such a cytogenetic abnormality would dominate the
clinical outcome rather than the developmental stage of the lymphoblasts. e clinical significance
of specific cytogenetic abnormalities in TPB ALL should be validated with further studies.
References
1. Koehler M, Behm FG, Shuster J, Crist W, Borowitz M, Look AT, et al. Transitional pre-B- cell acute
lymphoblastic leukemia of childhood is associated with favorable prognostic clinical features and an excellent
outcome: a Pediatric Oncology Group study. Leukemia. 1993; 7(12): 2064-8.
2. Lopez-Karpovitch X, Piedras J. Transitional pre-B cell acute lymphoblastic leukemia in adults. Am J Hematol.
1999; 61(4); 274-6.
Lymphoblasts Expressing Surface IGM in the Absence
of Kappa and Lambda in Philadelphia Positive Acute
Lymphoblastic Leukemia: Philadelphia Chromosome
Positive Transitional Pre-B Acute Lymphoblastic Leukemia
OPEN ACCESS
*Correspondence:
Merve Pamukcuoglu, Department of
Hematology, Gazi University Medical
Faculty, Turkey, Tel: +90 312 2025511;
Fax: +90 312 2236714;
E-mail: drmpamuk@yahoo.com
Received Date: 18 Sep 2017
Accepted Date: 05 Nov 2017
Published Date: 27 Nov 2017
Citation:
Pamukcuoglu M, Acar K, Sucak GT.
Lymphoblasts Expressing Surface
IGM in the Absence of Kappa and
Lambda in Philadelphia Positive Acute
Lymphoblastic Leukemia: Philadelphia
Chromosome Positive Transitional
Pre-B Acute Lymphoblastic Leukemia.
Clin Oncol. 2017; 2: 1369.
Copyright © 2017 Merve
Pamukcuoglu. This is an open access
article distributed under the Creative
Commons Attribution License, which
permits unrestricted use, distribution,
and reproduction in any medium,
provided the original work is properly
cited.
Letter to Editor
Published: 27 Nov, 2017
Merve Pamukcuoglu*, Kadir Acar and Gülsan Türköz Sucak
Department of Hematology, Gazi University Medical Faculty, Turkey