_____________________________________________________________________________________________________ *Corresponding author: E-mail: ernanefito@gmail.com; J. Adv. Biol. Biotechnol., vol. 27, no. 5, pp. 1-12, 2024 Journal of Advances in Biology & Biotechnology Volume 27, Issue 5, Page 1-12, 2024; Article no.JABB.114058 ISSN: 2394-1081 Azospirilla and Nitrogen Fertilization to Promote Maize Seed Development and Yield Marco Túlio G. de Paula a , Miguel Henrique R. Franco a , Roberta C. de Oliveira a , Regina Maria Q. Lana a , Beno Wendling a and Ernane M. Lemes a* a Instituto de Ciências Agrárias (ICIAG), Universidade Federal de Uberlândia (UFU), Uberlândia, Brazil. Authors’ contributions This work was carried out in collaboration among all authors. Author MTGDP conducted the experimental maintenance and data assessment. Author MHRF conducted the experimental supervision and intellectual contribution. Author RCDO conducted the experimental supervision and intellectual contribution. Author RMQL intellectual contribution. Author BW intellectual contribution. Author EML manuscript drift and critical review. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JABB/2024/v27i5757 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/114058 Received: 08/01/2024 Accepted: 13/03/2024 Published: 26/03/2024 ABSTRACT Aims: This study evaluated the interaction of Azospirillum brasilense (Ab) strains and nitrogen fertilizer doses on maize. Study Design: Laboratory experiment evaluated Ab strains (PCR and PTF) on maize seed germination and vigor (completely randomized design). Field experiment evaluated Ab strains and nitrogen fertilization on maize yield parameters (randomized block design). Place and Duration of Study: The germination and vigor experiment (10 days in April 2019) was implemented in a controlled environment at the Seed Analysis Laboratory of the Universidade Original Research Article