16 – 19 September 2017, Vienna, Austria Poster discussion hub abstracts Virtual Organ Computer-aided Analysis (VOCAL) technique. Logis- tic regression models using PET as the outcome were determined for each PVI. Covariates included: study group, age, BMI, smoking status, aspirin use, parity, material deprivation of area of residence and Mean Arterial Pressure (MAP). Results: The overall rate of PET was 12%. No significant differences in age, smoking status, deprivation or MAP were seen between groups. Significant differences in parity (P=0.002), BMI (P<0.001) and aspirin use (P<0.001) were noted. In the logistic regression model, a 1% increase in VI from a mean of 14.2 (SD 7.7) to 15.2% was associated with a 10% reduction in the odds of PET (OR 0.90, 95% CI 0.82-0.98, P=0.02) and a 1 unit increase in FI (mean 42.1, SD 10.3) was associated with a 6% reduction in the odds of PET (OR 0.94, 95% CI 0.89-1.0, P=0.04). A doubling in VFI (median 5.1, IQR 3.4-8.3) was associated with a 57% reduction in the odds of PET (OR 0.43, 95% CI 0.26-0.70, P=0.001). Conclusions: Using logistic regression, VI, FI and VFI were predictive of PET in separate models controlling for confounding variables. This technique may be a useful adjunct for clinicians wishing to refine first trimester screening models to predict PET. P21.05 Prediction of pre-eclampsia by biochemical and biophysical markers at first trimester among Mongolian pregnant women U. Tserensambuu Obstetrics and Gynecology Hospital, National Centre for Maternal and Child Health of Mongolia, Ulaanbaatar, Mongolia Objectives Screening and assessment of pre-eclampsia by PAPP-A, PlGF, MAP, and mPI at first trimester of gestation. Methods: The study conducted among 361 singleton pregnant women at 11-13 +6 weeks, who were visiting antenatal care services, between March, 2015 and January, 2016. The cross sectional study design was used to implement this study. The ethical norms of biomedical research were applied throughout the course of study. The PlGF, PAPP-A in plasma were identified by PerkinElmer kits, uterine artery pulsatility index measured by GE ultrasound machine, the blood pressure was measured twice, in each arm of pregnant woman, by Omron blood pressure monitor. Results: The PIGF level of the group with pre-eclampsia compli- cations was 38.6±19.6 pg/ml, in the group without pre-eclampsia level was 45.1±24.0 pg/ml (p=0.010), while PAPP-A concentra- tion in the group with pre-eclampsia was 366.1±195.3 mU/L and 633.6±496.9 mU/L (p=0.003) in the group without pre-eclampsia. MAP 80.1-100 mm Hg group had 20.6 times higher risk of pre-eclampsia in compare to MAP normal group (OR 20.6, CI 95% 2.8-152.1, p=0.003). No significant differences observed in mPI of both groups (p=0.222). The logistic regression analysis shows that pregnant women previous without pre-eclampsia but have MAP more than 80mm Hg, PAPP-A less than 330 mU/L and PIGF less than 35pg/ml or have three risk factors of pre-eclampsia (OR 8.12 p=0.018) have higher level of pre-eclampsia compared to women with 1 or 2 risk factors (OR 3.54-5.65). Conclusions: In the groups of pregnant women with pre-eclampsia, the levels of PlGF, PAPP-A are lower compared to those, who have no pre-eclampsia. The level of risk of pre-eclampsia for women without previous pre-eclampsia depends on the number of risk factors. P21.06 Perinatal risk degree determination: the role of ductus venosus flow, cerebroplacental ratio and cardiothoracic ratio I. Safonova 1 , I. Lukjanova 2 , O. Zhadan 2 , R. Safonov 3 1 Ultrasoud Diagnostics, Kharkiv, Medical Academy of Postgraduate Education, Kharkiv, Ukraine; 2 Radiology, SI ‘Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine’, Kyiv, Ukraine; 3 Kharkiv Regional Perinatal Centre, Kharkiv, Ukraine Objectives: The purpose of the study was to learn a role of cerebro-placental ratio and cardiothoracic ratio in the degree of perinatal risk determination. Methods: Antenatal US examinations were performed in 1448 fetuses with a high perinatal risk. The study included Doppler assessment of placental profile and fetal cardiovascular profile. Pregnancy outcomes and neonatal status were studied. Postnatal results were classified as adverse general postnatal outcome which meant perinatal or infant death and adverse clinical postnatal outcome which meant perinatal acidemia, Apgar score of 0–4 points, persistent pathological condition of the newborn. All the fetuses of the studied cohort were divided into two clinical groups: 638 fetuses with poor general postnatal outcome and/or clinical postnatal outcome (I group) and 810 fetuses with favourable clinical postnatal outcome (II group). Results: The most significant differences occurred at the frequency of registration ductus venosus (DV) a-reverse wave, p<0.01. The increased cardio-thoracic ratio (CTR) occurred in fetuses of group I 9 times more frequent than of group II. The cerebroplacental ratio (CPR) reduction occurred 18 times, the prevalence of reverse flow component of the fetal aortic isthmus 6,2 times and the deepening of ductus venosus myocardial isovolumetric relaxation phase in the group I were registered 21 times more often than in group II, however these differences did not have statistical significance due to the small number of observations. Conclusions: Taking into account the limited possibilities of single centre study should be considered as necessary further multicentre studies of the role of fetal CTR and CPR as predictors of adverse pregnancy outcomes, in order to include these aspects in obstetrical management protocols of high perinatal risk fetuses monitoring to determine the perinatal risk degree ant to choose the best point for delivery. P21.07 Association between uterine artery blood flow volume and fetal weight gain H. Arneberg 2 , T. Andersen 2 , T. Scholbach 3 , L. Loraas 1 , T.M. Eggebø 1 1 National Centre for Fetal Medicine, St Olavs Hospital, Trondheim, Norway; 2 Department of Laboratory Medicine, Children’s and Women’s Health, Norwegian University of Science and Technology, Trondheim, Norway; 3 Leipzig Ultrasound Institute, Leipzig, Germany Objectives: The aim of the study was to explore an eventual association between blood flow measured by PixelFlux in second trimester and fetal weight gain/day. Methods: We conducted a prospective cohort study in 47 women with single pregnancies in week 24. PixelFlux is a new method based on pixelwise calculation of spatially angle-corrected velocities and areas of all pixels inside a vessel. Velocity is coded by colour, and by comparing the colour of each pixel in the cross-sectional area with the preset colour bar, it is possible to measure blood flow velocities directly. Then, all pixels’ flow volumes inside a vessels section are added to calculate the flow volume of the vessel during a heart cycle. Fetal weight in the second trimester was estimated © The Authors 2017 © Ultrasound in Obstetrics & Gynecology 2017; 50 (Suppl. 1): 154–256. 223