58 J. Appl. Emerg. Sci., 2018, 8(1) Parametric Effect of Distillery Effluent as Substrate in Microbial Fuel Cell for Power Generation Mohammad Siddique 1 , Mohammmad Najam Khan 1 , Abdul Sattar Jatoi 2 , Shaheen Aziz 3 , Sohail Ahmed Soomro 3 1 Department of Chemical Engineering, Faculty of Engineering and Architecture, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan, 2 Department of Chemical Engineering Dawood University of Engineering & Technology, Karachi, Pakistan, 3 Department of Chemical Engineering, Mehran University of Engineering & Technology, Jamshoro, Pakistan Abstract Depletion of fossil fuel increased direction towards renewalable energy sources. Microbial Fuel Cell substitute technology for converting waste water from industry effluent as well meet the requirement for environmental problem. Study was made to treat the distillery effluent as substrate in mfc for bio-energy considering effect of oxygen flow rate, pH and substrate concentration. Different organic load were used in mfc for power generation and different oxygen flowrate for oxidation of proton coming from anode chamber effect of pH also keep consideration regading microbial growth of saccharomyces as a biocatalyst. The maximum power generation were observed at optimized condition of oxygen flow rate 250rpm, pH6 and substrate 60% (in form of organic load) at 0.9114 mW and current density 82.48 mA/m 2 . Keywords: Distillery; Effluent; Substrate; Power Generation; MFC Corresponding author’s email: siddiqnasar786@gmail.com INTRODUCTION The growing demand for energy, depletion of fossil fuels and increasing concerns of environmental issues have challenged researchers to develop new technological processes to generate clean and sustainable energy mainly through the utilization of renewable energy sources (Cai et al., 2013; Jatoi et al., 2016c; Jatoi et al., 2018; Liu et al., 2014; Logan et al., 2006). Recently, microbial fuel cell (MFC) technology has emerged as a promising sustainable technology to meet increasing energy demand that can utilize organic materials as a fuel (Pant et al., 2010; Yusoff et al., 2013) . MFCs are bio-electrochemical devices capable of converting biochemical energy into electricalenergy through the catalytic reaction of microorganisms (Yusoff et al., 2013). MFCs have remarkable electron-donor versatility as the microbes usewastewater as substrates to generate electricity and simultaneously accomplish wastewater treatment (Jatoi et al., 2016a; Jatoi et al., 2016b; Lu et al., 2009; Oh and Logan, 2005; Pant et al., 2010; Parkash et al., 2015; Soomro et al., 2016). Production of unmanageable quantity of sewage sludge from wastewater treatment plants is the major issue in terms of capitaland environmental burden. This costs 60% of the total plant capital cost, and its disposal has become problematic due to stringent sludge disposal laws (Canales et al., 1994; Pilli et al., 2011; Xiao et al., 2013). MFC technology provides new opportunities for the sustainable wastewater treatment by converting waste into energy, which may offset the operational costs of wastewater treatment plant (Lu et al., 2009). High concentrations of organic matter, mainly protein and carbohydrate can be found in sewage sludge (Wang et al., 2006; Xiao et al., 2013). Wang et al. (2006) reported the total protein and carbohydrate in sludge to be 12,036 mg/L and 2109 mg/L respectively. However, it is known that generation of power during the MFC process might be influenced by the efficient degradation of biomass in MFCs (Bougrier et al., 2008). In this study distillery effluent used as substrate in mfc for power generation. MATERIALS AND METHODS Microorganism Yeast S.cerevisiae M-9 (Shah et al., 2010) were purchase from local market with analytical grade.