ABR Vol 6 [3] May2015 136 | P age ©2015 Society of Education, India Advances in Bioresearch Adv. Biores., Vol 6 (3) May 2015: 136-143 ©2015 Society of Education, India Print ISSN 0976-4585; Online ISSN 2277-1573 Journal’s URL:http://www.soeagra.com/abr.html CODEN: ABRDC3 ICV 7.20 [Poland] ORIGINAL ARTICLE Determination on Growth indices, Blood parameters, Immune responses and Intestine bacterial flora of Bester juvenile fish (Huso huso×Acipenser ruthenus) Fed by Biomin Nutritional supplements Fariborz Taghavi 1* , Mehrdad Nasri Tajan 2 , Abbas Ali Zamini 3 1 Department of Aquaculture, Rasht Branch, Islamic Azad University, Rasht, Iran 2 Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali, Iran 3 Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran ABSTRACT This study is evaluated the effects of Biomin P.E.P supplement on growth indices, blood and immune factors, and intestine bacterial flora of Bester juvenile fish. The survey is conducted on Bester juvenile sturgeons in fisheries laboratory located in Islamic Azad University Science and Research of Guilan Branch in the fall of 1392. The completely random scheme of the research includes 4 treatments each of which has 3 repetitions. The supplement was added to the basal diet on 4 levels of 0, 1, 1/5, and 2 g / kg.72 of 45-g Bester fish were fed for 60 days based on 5 percent of the body weight in 12 tanks (100-liter) that 6 fish in each tank. At the end of the farming period some of the growth indices, blood and immune factors, and intestine bacterial flora were compared. So that in treatment 2 and in the level of 1/5 g/kg in some growth and nutrition factors such as average initial weight, total weight, total length, FCR, SGR, PBWI, BWI, ADG, at the end of the period, a significant difference was observed between treatment and control (P<0.05). But in the factors like Average initial length and Condition Factor(K), there was no statistically significant differences between treatment and control at the end of the period (P>0.05). Evaluation of blood factors indicated that the rate of red blood cells (RBC), white blood cell(WBC), hemoglobin (Hb), hematocrit (HCT), neutrophils, has decreased by increasing amounts of Biomin P.E.P compared to the control, and showed significant statistically difference (P<0.05). In evaluation of biochemical and immune factors, the amount of total immunoglobulin, albumin, total protein with different amounts of Biomin P.E.P had significant statistically difference (P<0.05).Therefore, this supplement affects the blood biochemical factors and can influence on their recovery. The total count of bacterial flora in the dilution TSA 10 -1 showed that the levels of 1 and 1/5 g/kg has less amount compared to the control, and in MRS10 -1 at the level of 1 g/kg LAB bacteria were higher compared to control, and it can be said with reducing bacteria on TSA, LAB bacteria have been replaced. it is therefore suggested of 1/5 g Biomin Nutritional Supplement in the 1 kg diet. Keywords: Bester Sturgeon (Huso huso×Acipenser ruthenus), Biomin P.E.P, Intestine bacterial flora, Growth, Blood and Immune responses. Received 10/02/2015 Accepted 29/04/2015 ©2015 Society of Education, India How to cite this article: Fariborz T, Mehrdad N T, Abbas A Z. Determination on Growth indices, Blood parameters, Immune responses and Intestine bacterial flora of Bester juvenile fish (Huso huso×Acipenser ruthenus) Fed by Biomin Nutritional supplements. Adv. Biores., Vol 6 [3] May 2015: 136-143. DOI: 10.15515/abr.0976-4585.6.3.136143 INTRODUCTION In recent years with reducing Sturgeons fishing in the Caspian Sea, their fattening cultivation has achieved strong motivation in the world, including Iran. As today in many parts of the world containing Iran, meat production and the possibility getting larvae from natural spawners and thus Forming sturgeon breeding herds in soil pools, fiberglass tubs and concrete tanks is provided [1].Today scientists believe that the only way to save valuable resources of Sturgeon is to fulfill research and executive activities with the aim of maintaining and restocking resources and development the technology of breeding sturgeon in breeding environments, preventing illegal fishing, and cooperation with international organizations to protect biodiversity and endangered species. Scientific studies show that the efficiency of feeding, feeding rate, water temperature and fish size are the economic factors that Advances in Bioresearch