How to cite (Vancouver). Yarim FG, Pekmezci D, Yarim M, Yagci BB, Gokceoglu A, Sozmen M, et al. Efects of amitraz plus-Parapoxvirus ovis on EGF, VEGF, IGF-1 and IGF-2 in canine generalized demodicosis. Rev MVZ Cordoba. 2022; 27(3):e2619. https://doi.org/10.21897/rmvz.2619 2022; september-december. 27(3):e2619. https://doi.org/10.21897/rmvz.2619 Journal MVZ Cordoba Original ISSNL: 0122-0268 ©The Author(s) 2021. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons. org/licenses/by-nc-sa/4.0/), lets others remix, tweak, and build upon your work non-commercially, as long as they credit you and license their new creations under the identical terms. Effects of amitraz plus Parapoxvirus ovis on EGF, VEGF, IGF-1 and IGF-2 in canine generalized demodicosis F. Gul Yarim 1 * ; Didem Pekmezci 2 ; Murat Yarim 3 ; B. Bugrahan Yagci 4 ; Ayris Gokceoglu 1 ; Mahmut Sozmen 3 ; G. Zafer Pekmezci 5 ; Efe Karaca 3 ; Murat Guzel 2 . 1 Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Biochemistry, Samsun, Turkey. 2 Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Internal Medicine, Samsun, Turkey. 3 Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Pathology, Samsun, Turkey. 4 Kirikkale University, Faculty of Veterinary Medicine, Department of Internal Medicine, Kirikkale, Turkey. 5 Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Preclinical Sciences, Samsun, Turkey. *Correspondence: gulyarim@omu.edu.tr Received: November 2021; Accepted: July 2022; Published: September 2022. ABSTRACT Objective. The purpose of the study is to investigate the efect of treatment with amitraz plus- Parapoxvirus ovis (IPPVO) on serum concentrations and skin expressions of insulin-like growth factor (IGF)-1 and -2, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), in dogs sufering from generalised demodicosis (GD). Materials and Methods. GD afected dogs were injected 1 ml IPPVO on days 0, 2 and 9 subcutaneously in addition to amitraz (0.025%) treatment twice weekly for 80 days. IGF-1, IGF-2, EGF and VEGF concentrations in blood serum were measured by canine-specifc enzyme-linked immunosorbent assay kit. EGF, VEGF, IGF-1, and IGF-2 expressions in skin biopsy specimens were examined immunohistochemically. Results. After the treatment of the dogs with amitraz plus-IPPVO in GD, we demonstrated a signifcant reduction in both circulating concentrations and skin expressions of EGF, VEGF, IGF-1, and IGF-2, which have a role in preserving skin integrity and wound healing. Conclusions. Results of this study suggest that IGF-1, IGF-2 EGF, and VEGF have a crucial role in the progression of GD in dogs. It is believed that the fndings from this study will contribute to the development of new strategies for the treatment of GD, which is an important health problem for dogs. Keywords: Demodex canis; dog; generalized demodicosis; growth factor; skin (Source: MeSH). RESUMEN Objetivo. El propósito del estudio es investigar el efecto del tratamiento con amitraz más- Parapoxvirus ovis (IPPVO) sobre las concentraciones séricas y las expresiones cutáneas del factor de crecimiento insulínico (IGF) -1 y -2, factor de crecimiento epidérmico (EGF), vascular factor de crecimiento endotelial (VEGF), en perros que padecen demodicosis generalizada (GD). Materiales