© 2022 JETIR July 2022, Volume 9, Issue 7 www.jetir.org (ISSN-2349-5162) JETIR2207688 Journal of Emerging Technologies and Innovative Research (JETIR) www.jetir.org g673 Evaluation of Preliminary Phytochemical, Antimicrobial and Anthelmintic activity of Zizyphus oenoplia (L) Mill Lokmanya Tilak Institute of Pharmaceutical Sciences,Pune. Hrutuja Wagh * , Dhanashree Dupade, Dr. Meera Deshmukh ABSTRACT Only fewer medicinal plant shows Antimicrobial and Anthelmintic property in which Zizyphus oenoplia is one of them. The aim of present study was to investigate the preliminary phytochemical constituent and Antimicrobial and Anthelmintic activity of Zizyphus oenoplia against Microbes (bacterial suspention) and earthworm (Pheretima postuma). The concentrations (100, 200, 300 & 400 mg/mL) of each extracts were studied in activity, which involved the determination of Minimum Inhibitory Concentration by bacteria ,time of paralysis and time of death of the worms. Antimicrobial activity done by Disk diffusion Method and Broth Dilution Method . The total alcoholic extract and its ethanolic and aqueous fraction exhibited anthelmintic activity at highest concentration of 400 mg/mL. Albendazole in same concentration as that of extract was included as standard reference and normal saline water as control. So it could be confirmed that the total alcoholic extract, ethanol fraction and aqueous fraction of root of Zizyphus oenoplia showed significant anthelmintic properties comparable with standard drug, which is effective against parasitic infection of humans. Key words: Zizyphus oenoplia, MIC, Antimicrobial, Anthelmintic property INTRODUCTION Many drugs commonly used today are of herbal origin. Indeed, about 25 percent of the prescription drugs dispensed in the United States contain at least one active ingredient derived from plant material. Some are made from plant extracts; others are synthesized to mimic a natural plant compound. The US herbal medicine consumption alone was worth US$ 17 billion in the year 2000 and the global market for herbal medicines today is estimated to be a whopping US$ 60 billion. The World Health Organization (WHO) estimates that 4 billion people, 80 percent of the world population, presently use herbal medicine for some aspect of primary health care. Major pharmaceutical companies are currently conducting extensive research on plant materials gathered from the rain forests and other places for their potential medicinal value.