IJMSDH, (2024) PageNo.110-120 www.ijmsdh.org IJMSDH 110 Volume 10, Issue 10, October 2024, Publish Date: 21-10-2024 Doi https://doi.org/10.55640/ijmsdh-10-10-13 BIOCHEMICAL AND HISTOPATHOLOGICAL ANALYSIS OF AQUEOUS EXTRACT OF AN APHRODISIAC FORMULATION AGAINST ACETAMINOPHEN INDUCE HEPATOCYTE INJURIES IN WISTAR RATS KABRUK CAROLINE EZIAFAKAEGO 1, 2 , AMUZAT ALIYU OLALEKAN 1 , IBRAHIM MOHAMMED IBRAHIM 1, 3 , YAKUBU NDATSU 1 1 Department of Biochemistry, Faculty of Natural Sciences, Ibrahim Badamasi Babangida University, Lapai, Niger State of Nigeria. 2 Federal polytechnic, Bida, Niger State. 3 Sheda Science and Technology Complex, Abuja. ABSTRACT The aimed of this study was to examine the biochemical parameters and histopathological effects of aqueous extract of an aphrodisiac formulation against acetaminophen induce hepatocyte injuries in Wistar rats. Zainacin Dadin duniya (aphrodisiac herbs) extract was prepared by cold maceration. The animal study was done using 30 adults both male and female Wistar rats was grouped into 5 groups (n=6) and orally administered with five different treatments (saline, liver drug, acetaminophen, extract and extract/ acetaminophen) for the period of 28days. The blood is collected from each grouped rat for biochemical parameters test and all rats were later sacrificed for histologically processed investigation. The results showed that the oral administration of the acetaminophen in group 3 treated rats causes a significant (P<0.05) increased in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), but, when administered with the extract in group 4 rats, there was a significantly (p<0.05) decreased in the activities of ALT, AST and ALP. Significantly (p<0.05) increased in the Kidney Function Markers (Creatinine, and Urea) and decreased in Glucose level in the group 3 rats treated with acetaminophen, when compared with the group 4 rats treated with the extract, there was a significant (p<0.05) decreased in Creatinine, and Urea level and increased in the Glucose level. The serum total protein, Albumin, and Globulin significantly (p<0.05) decreased in the group 3 rats treated with acetaminophen while the bilirubin increased significantly. While group 4 rats showed significant increase in the total protein, Albumin, and Globulin and decreased in the bilirubin levels. The Results of Histopathology findings revealed hepatic tissue with distorted hepatocytes, portal vein, and sinusoids accompanied with massive necrosis in the liver of the group 3 rats treated with acetaminophen. While the liver of the group 4 rats showed, hepatic tissue with preserved architecture composed of cords of normal hepatocytes, normal portal vein, and sinusoids. There are no features of acute or chronic damage. The histologically Results of the small intestines of the all five (5) grouped rats showed preserved layers of muscosa, submucosa, muscularis externa, and serosa. There are no features of acute or chronic toxicity. Conclusively, the prolonged oral consumption International Journal of Medical Science and Dental Health (Open Access)