How to cite (Vancouver). Vargas-Abella JC, Tarazona-Manrique LE, Andrade-Becerra RJ. Serotyping and antimicrobial resistance profle of Salmonella spp. isolated from crocodilians in captivity. Rev MVZ Cordoba. 2023; 27(1):e3045. https://doi.org/10.21897/rmvz.3045 2023; january-april. 28(1):e3045. https://doi.org/10.21897/rmvz.3045 Journal MVZ Cordoba Original ISSNL: 0122-0268 ©The Author(s) 2023. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons. org/licenses/by-nc-sa/4.0/), lets others remix, tweak, and build upon your work non-commercially, as long as they credit you and license their new creations under the identical terms. Serotyping and antimicrobial resistance profile of Salmonella spp. isolated from crocodilians in captivity Julio César Vargas-Abella 1 ; Luis Edgar Tarazona-Manrique 1 * ; Roy José Andrade-Becerra 1 . 1 Universidad Pedagógica y Tecnológica de Colombia. Facultad de ciencias agropecuarias. Escuela de medicina veterinaria y zootecnia. Grupo de investigación en salud y producción animal del trópico alto colombiano- GIPATRACOL. Laboratorio de microbiología veterinaria. Línea de investigación en especies silvestres y promisorias. Tunja. Boyacá. *Correspondencia: luis.tarazona01@uptc.edu.co Received: September 2022; Accepted: December 2022; Published: January 2023. ABSTRACT Objective. Determine the presence of Salmonella spp and the AMR in isolates of cloacal and muscle swabs in specimens of Caiman crocodilus fuscus and Crocodylus acutus in captivity, in Colombia. Materials and methods. A cross-sectional and descriptive sampling was carried out with a non- probabilistic design for convenience, once, during the month of February 2021. 150 Caiman crocodilus fuscus and Crocodylus acutus animals were included, with a 50/50 distribution. Cloacal and muscle samples were taken with sterile swabs. These were planted on MacConkey agar, then on SS agar. These positive samples were then reconfrmed on TSI agar. Serotyping was performed with specifc monovalent and polyvalent antisera. The difusion test was performed on Mueller-Hinton agar. Results. There was no growth of colonies in any muscle sample. The presence of the bacteria in cloacal samples of Caiman crocodilus fuscus was 36% and for Crocodylus acutus 50.6%. The serotypes with the highest detection percentage in both groups were serogroup C1 and polyvalent serogroup B. Some colonies were resistant to tetracycline, while other colonies had intermediate resistance to ampicillin, cefoxitin, sulfa trimethoprim, and tetracycline. Conclusions. This is the frst report related to the detection of Salmonella spp. in alligator carcasses in the country, without fnding the presence of the agent in any sample evaluated, however, the importance of continuing its monitoring is emphasized to guarantee innocuous products Keywords: Reptiles; antimicrobial resistance; foodborne diseases; meat quality (Source: MeSH). RESUMEN Objetivo. Determinar la presencia de Salmonella spp y el PRA en aislamientos de hisopados cloacales y musculares en especímenes de Caiman crocodilus fuscus y Crocodylus acutus en cautiverio en Colombia. Materiales y métodos. Se realizó un muestreo de tipo transversal y descriptivo con un diseño no probabilístico por conveniencia una única vez, durante el mes de febrero de 2021. Se incluyeron 150 animales de Caiman crocodilus fuscus y Crocodylus acutus, con una distribución