with Ms (R 0.355, p<0.001). For the FG only an association with olive oil was observed (R 0.179, p¼0.047) -g/day-. The N associated with the Ms were: total carbohydrates (R 0.218; p¼0.015), total fat (R 0.241, p¼0.007), MUFA (R 0.499, p¼0.005), PUFA (R 0.288, p¼0.011), linoleic acid (R 0.200, p¼0.026), linolenic acid (R 0.177, p¼0.049), alcohol (R 0.398, p<0.001) -g/ day-, total Kcal (R 0.290, p¼0.001), % protein Kcal (R 0.298, p¼0.001) and phytosterols (R 0.238, p¼0.008) -mg/day-. After adjusting each one of the N/E with age and sex, no significant association was obtained. Sex was the greatest predictor of Ms (beta coefficient -woman vs man- - 15.20 (95% CI: 21.64; 8.75; p<0.001). Conclusions: It has not been possible to clearly demonstrate an associa- tion between daily consumed nutrients and Ms with the univariate anal- ysis adjusted for age and sex in the described observations. The relationships between feeding and clinical events are complex and require precise analyses to avoid errors that could make reference to hypotheses of reverse causality. Disclosure of interest: M. Ortiz: None declared, M. Torrego : None declared, A. Barabash: None declared, M.  A. Rubio: None declared, A. Lar- rad: None declared, G. Hern andez : None declared, C. Moreno: None declared, M. Gonz alez: None declared, R. Cabrera: None declared, P. Matía Grant/Research Support from: PI 17/01732 IP P Matia; FEDER; ISCIII. MON-P318 BARIATRIC SURGERY: METABOLIC RESULTS AND COMPLICATIONS TO 5 YEARS M. Martínez 1, * , C. Palomares 1 , L. Martinez 2 , A. Fornovi 3 , E. Parre~ no 4 , R. Ballester 2 , J. Martinez 5 , M.P. Guillen 5 , E. Arjonilla 6 , M.J. Hernandez 7 . 1 Endocrinologia y Nutrici on, HospitalC Comarcal Del Noroeste, Caravaca de la Cruz, Spain; 2 Endocrinologia y Nutrici on, Hospital Virgen Del Castillo, Yecla, Spain; 3 Endocrinologia y Nutrici on, Hospital Vega Baja, Orihuela, Spain; 4 Endocrinologia y Nutrici on, Hospital de La Vega Lorenzo Guirao, Cieza, Spain; 5 Cirugía, Hospital Comarcal Del Noroeste, Caravaca de la Cruz, Spain; 6 Endocrinologia y Nutrici on, Hospital Morales Meseguer, Murcia, Spain; 7 Medicina Interna, Hospital General Universitario Santa Lucia, Cartagena, Spain * Corresponding author. Rationale: Assess changes in weight, comorbidities and complications after 5 years of a gastric bypass. Methods: We present a retrospective observational study that includes 113 obese patients who underwent laparoscopic gastric bypass between 2007 and 2012 in Morales Meseguer Hospital. Results: The study population included 113 obese patients (76.1%\vs 23.9%_) with an average age of 42.1±10.1 years old and a mean weight before surgery of 126.4±20.4 kg (BMI: 47.5±6.1 kg/m2). The arterial hy- pertension (AH) was present in 46.8% of the patients, dyslipidemia in 19.8%, type 2 Diabetes Mellitus (DM2) in 17.1% and Sleep Apnea-Hypopnea Syndrome (SAHS) in 22.5%. Six months after surgery, the percentage of lost excess weight was 52.4±15.8% (BMI of 34.5±5.4 kg/m2), 2 years after surgery was 69.9±18.9% (BMI of 29.9±5.4) and 5 years after surgery was 58.5% ± 22.3 (BMI of 32.7±6.1). All comorbidities improved 5 years after surgery, this improvement was statistically significant for AH (46.8 vs 27.8%, p<0.001), DM2 (17.1 vs 8.9%, p<0.001) and SAHS (22.5 vs 9.1%, p<0.001). Although without statistical significance, dyslipidemia also improved (17.1 vs 11.4%, p¼0.4). The ma- jority of patients presented some nutritional deficit that needed treat- ment: iron in 71.6%, vitamin D in 40.8%, vitamin B12 in 31.6% and folic acid in 28.6%. There were surgical complications in 36 patients, 22 of these cases were early complications (13 hemorrhages, 6 infections and 3 anastomosis leaks) and 12 were late complications (6 ulcers and 6 anastomotic steno- sis), however all of them were resolved successfully. Conclusions: Gastric bypass is a safe and effective technique in the treatment of obesity. In our population, the percentage of lost excess weight 5 year after surgery is 58.5%, in addition, they improve all comor- bidities without serious surgical complications and without any case of death. Although nutritional deficits are frequent, they do not represent any clinical problem. Disclosure of interest: None declared. MON-P319 FUNCTIONALITY AND DENSITY MITOCHONDRIAL OF SUBCUTANEOUS ADIPOSE TISSUE IN MEN WITH HIV-ASSOCIATED LIPODYSTROPHY SYNDROME M.P. Guimar~ aes 1, * , A.O. Souza 2 , J.G. Maciel 1 , M.P. Pupim 1 , N.L.S. Monteverde 1 , M. Nogueira-Barbosa 1 , L.C. Alberici 2 , R.C. Santana 1 , A.M. Navarro 1 . 1 Departament of Internal Medicine, University of Sao Paulo, Ribeirao Preto, Brazil; 2 Department of Physics and Chemistry, University of Sao Paulo, Ribeirao Preto, Brazil * Corresponding author. Rationale: The effectiveness of antiretroviral therapies (ART) increased the life expectancy of HIV-infected patients. However, ART and HIV itself are associated with mitochondrial toxicity that affects systemic metabolism and contribute with metabolic and morphologic changes observed in HIV- associated lipodystrophy syndrome (HALS). The aim of this study was to evaluate the functionality and density mitochondrial of subcutaneous adipose tissue in men with HALS. Methods: We evaluated men seropositive and seronegative for HIV. The volunteers were divided into three groups: HALS (HIV+ with lipodys- trophy); HALS- (HIV+ without lipodystrophy) and CTL (HIV-). We measured: weight, BMI, waist circumference (WC), body composition by DXA, glucose and lipid metabolism. For mitochondrial respiration, the samples of the dorsocervical region were collected and evaluated by Oxygraph-2k. The states of respiration evaluated were: Phosphorylation (State P), Leak (State L) and Uncoupled (State E). The mitochondrial density was evaluated by citrate synthase activity. Data were processed using SPSS, p<0.05. Results: We evaluated 9 HALS+, 6 HALS, and 9 CTL. The mean age was 48.8 (±6.7) years and BMI was 24.0 (±2.9) Kg/m2. There was no difference between groups in BMI, WC (cm), %FM, and FFM (kg). All individuals HIV+ were clinically stable and had undetectable viral load. There were no sig- nificant differences in the mitochondrial rations as P/L (0.57±0.14 versus 0.62±0.2 versus 0.65±0.38), L/E (0.27±0.16 versus 0.3±0.13 versus 0.25±0.11), and P/E (2.84±2.15 versus 2.35±0.96 versus 2.76±1.56) be- tween HALS+, HALS-, and CTL groups, respectively. Conclusions: In this study, we did not find changes in the functionality and density mitochondrial of dorsocervical adipose tissue in men with HALS. Disclosure of interest: None declared. MON-P320 CHANGES IN PLASMA VITAMIN D LEVEL AND ANTHROPOMETRIC MEASUREMENTS AFTER A HIGH-INTENSITY INTERVAL TRAINING IN OVERWEIGHT MALE ADOLESCENTS M. Gholamalizadeh 1, * , S. doaei 2 , Z. Jamshidi 3 , R. Haghshenas 4 . 1 Cancer Research Center, Medical University of Shahid Beheshti, Iran; 2 Public Health, Department of Public health, North Khorasan University of Medical Sciences, Bojnurd, Tehran, Iran; 3 Medical University of Shahid Beheshti, Iran; 4 Sport Physiology Department, Semnan University, Semnan, Islamic Republic of Iran * Corresponding author. Rationale: The effect of a high-intensity interval training on plasma vitamin D levels and anthropometric measurements is not well-known. This study aimed to examine the effect of high-intensity interval training (HIIT) training on plasma level of vitamin D and anthropometric mea- surements in overweight male adolescents. Methods: 96 overweight students aged 12 to 16 years from two high schools participated in the study. The schools were randomly assigned to intervention and control groups. During the intervention, HIIT was per- formed 3 days/week for 6 weeks while participants in the control group were asked to walk at the same time. Anthropometric measurements and blood sample were obtained at baseline and after 6 weeks. Height, weight, and body mass index (BMI) were measured and Bioelectrical impedance analysis (BIA) was used to estimate percentage of body fat (%BF) and Abstracts / Clinical Nutrition 37 (2018) S46eS314 S263