Macedonian pharmaceutical bulletin, 68 (2) 47 - 51 (2022) ISSN: 1857-8969 DOI: 10.33320/maced.pharm.bull.2022.68.02.005 Original scientific paper Overview of the compendial methods for quantitative determination of glucose in solutions for intravenous infusion Shkendije Imeri 1 *, Snezana Zaharieva 1 , Gordana Majnova 2 , Suzana Angelova 2 , Jelena Acevska 3 , Katerina Brezovska 3 , Vasil Karchev 3 , Aneta Dimitrovska 3 1 Genaral Hospital, Kumanovo, October 11 th bb, 1300 Kumanovo, R. North Macedonia 2 PHI Center for Public Health Kumanovo, October 11 th bb, 1300 Kumanovo, R. North Macedonia 3 Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Thereza 47, 1000 Skopje, R. North Macedonia Received: October 2022; Accepted: December 2022 Abstract This paper provides a review of the pharmacopoeial methods for determination of the content of glucose in parenteral solutions (HPLC/RID method, polarimetry and volumetric titration) and evaluation of all critical aspects of the proposed methods, with a focus on accuracy, precision, time of analysis and resources for introducing, maintenance and conducting analysis. Comparative analysis of the results from assay (ten independent determinations) of Glucose 5% w/v solution for intravenous infusion, obtained using all three methods, was performed. The analysis confirmed that there is no statistical difference between the testing results obtained with each method, indicating that all three methods provide valid accurate and precise results. However, the availability of the equipment and trained personnel may be the crucial factor for small laboratories for selection of the method for assay of glucose in parenteral preparations. The obtained results and discussion on all critical aspects (accuracy, precision, time of analysis and resources for introducing, maintenance and conducting analysis) of the proposed methods, provide a valuable input to small laboratories on the suitability of these methods for quality control of Glucose 5% w/v solution for intravenous infusion. Key words: Glucose, HPLC/RID, Polarimetry, Volumetry Introduction Glucose is (+)-d-glucopyranose, derived from starch, which can be anhydrous or hydrated with one molecule of water and is widely used as an active substance in solutions for intravenous infusion or as an excipient in different pharmaceutical preparations, especially in preparations for parenteral use. Solutions for intravenous infusions containing glucose are available at two different concentrations 50 g/L and 100 g/L in containers with different volumes 50 mL, 100 mL, 500 mL and 1000 mL. Preparations for intraperitoneal use contain glucose in varying concentrations, ranging from 25 to 250 mmol/L (McMillin, 1990). European pharmacopoeia (Ph.Eur.), British pharmacopoeia (BP) and United States pharmacopoeia (USP) describe different monographs for quality control of glucose depending of the intended use. The determination of the content of glucose in the active substance and in the finished pharmaceutical preparations is a critical parameter for quality control. The pharmacopoeial monographs describe different methods for quantitative determination of glucose including cation - exchange liquid chromatography (LC) with __________________ *email: shkendijeimeri3@gmail.com