International Journal of Ayurvedic Medicine, Vol 14 (3), 2023; 717-723 Published online in http://ijam.co.in ISSN No: 0976-5921 Research Article Keywords: UV- visible spectrophotometric method, Vinpocetine, Validation, Marketed formulations, Stability study, Periwinkle. Introduction Vinpocetine (ethyl-apovincamine, 14,- ethoxycarbonyl-(3alpha, 16alphaethyl)-14, 15- eburnamine) is perhaps the most well-known nootropic agent (1). Vinpocetine was first synthesized from the vincamine alkaloid about forty years ago in the late 1960s. It was extracted from the leaf of the periwinkle plant (Vinca minor) (Figure 1). Other compounds available in the vinca alkaloid family include vintoperol, vinburnine and brovincane (2,3). After its synthesis in Hungary, the nootropic and neuroprotective properties of this drug were discovered, and from thereafter it appeared under the name Cavinton in 1978 and has been used widely in over 40 countries including Germany, Hungary, Japan, Russia and Poland for prevention and treatment of stroke and other cerebrovascular-related disorders. Several clinical studies have confirmed the neuroprotective properties of this drug (4,5). Vinpocetine (VPN) is a vincamine alkaloid derivative (Figure 2). It's mostly used to treat neurological illnesses including Alzheimer's and Parkinson's, as well as to increase brain blood flow (6,7). Vinpocetine works by inhibiting the enzyme phosphodiesterase type-1, which enhances cerebral blood flow specifically (8). Vinpocetine facilitates blood flow redistribution to ischemic regions and improves oxygen uptake and cerebral circulation (9). Because of its extensive first-pass metabolism, sluggish dissolution rate, poor water solubility, and limited absorption, Vinpocetine's bioavailability is extremely low. It also has a fast clearance rate and a short half-life, which leads to frequent drug administration (three times per day), which improves toxicity, and patient compliance (10,11). Development and Validation of UV-Spectrophotometric Method for Estimation of Vinpocetine in Marketed Formulation and Nanoformulation Chethan Kumar H B 1 , Bhaskar Kurangi 1* , Moazzim Soudagar 1 , Supriya Chimgave 2 , Swapnil Patil 1 1. Department of Pharmaceutics, 2. Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India. Abstract A quick, accurate, and cost-effective UV spectroscopy method was developed to estimate the Vinpocetine concentration in bulk, tablet dosage formulations and niosomes formulations, using a solvent ratio of (6:4) methanol: water. According to ICH guidelines, the proposed technique was validated and developed. In spite of linearity, precision, accuracy, specificity, Limit of Detection(LOD), and Limit of Quantification(LOQ), like parameters were validated by using UV/visible spectroscopy technique to analyze a spiked Vinpocetine solution. The wavelength at which the drug's maximum absorbance peak was obtained at 274 nm and the solvents used as methanol: water (6:4 w/v). The ethanol injection technique was used to prepare niosomes to analyze Vinpocetine in UV / visible spectrophotometric method. During the inter and intra-day studies, it was discovered that the developed UV technique was accurate, with % relative standard deviation ranging from 0.27 to 0.46 and 0.26 to 0.46, respectively. Vinpocetine overall recovery percentage was discovered to be between 98.42 to 99.82 %. LOQ and LOD were calculated to estimate the method's sensitivity, and they were observed to be 0.4565 µg/ml and 0.1506 µg/ ml, respectively. The estimation of Vinpocetine content in bulk form, marketed formulations and niosomes was achieved using the developed methodology. : A quick, accurate, and economical UV spectrophotometric method has been developed that estimates the vinpocetine concentration in bulk, tablet dosage formulations, and niosomes formulations. Figure No 1: Vinpocetine (periwinkle) plant Figure No 2: Chemical structure of Vinpocetine 717 * Corresponding Author: Bhaskar Kurangi Associate Professor, Department of Pharmaceutics, KLE College of Pharmacy, KLE Academy of Higher Education and Research, Belagavi. Karnataka. India. Email Id: bhaskarkurangi19@gmail.com