_____________________________________________________________________________________________________ *Corresponding author: E-mail: aaimam.bch@buk.edu.ng; Journal of Pharmaceutical Research International 20(2): 1-16, 2017; Article no.JPRI.38137 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) In vivo and in vitro Toxicity Studies of Crude and Partially Purified Leaf Extracts of Jatropha curcas in Wistar Albino Rats A. Mahe 1 , Y. Y. Muhammad 2 , A. Ibrahim 2 , A. Muhammad 3 , I. K. Adam 1 , M. K. Atiku 2 and A. A. Imam 2* 1 Department of Biochemistry, Faculty of Science, Federal University Dutse, Jigawa State, Nigeria. 2 Department of Biochemistry, Faculty of Biomedical Sciences, Bayero University, Kano, Nigeria. 3 Department of Pure and Applied Chemistry, Faculty of Science, Bayero University, Kano, Nigeria. Authors’ contributions This work was carried out in collaboration among all the authors. Authors AAI, A Mahe and A Muhammad designed the study and performed the experiments, statistical analysis and wrote the first draft of the manuscript. Authors AI, YYM, MKA and IKA supervised data collection and reviewed and made corrections to the manuscript. All authors read and approved the final draft of the manuscript. Article Information DOI: 10.9734/JPRI/2017/38137 Editor(s): (1) Jongwha Chang, University of Texas, College of Pharmacy, USA. Reviewers: (1) Afagnigni Alian Desire, University of Yaounde I, Cameroon. (2) Sangeetha Arullappan, Universiti Tunku Abdul Rahman & Jalan Universiti, Malaysia. (3) Md. Jakaria, Konkuk University, South Korea. Complete Peer review History: http://www.sciencedomain.org/review-history/22407 Received 14 th November 2017 Accepted 12 th December 2017 Published 22 nd December 2017 ABSTRACT Aims: Jatropha curcas (J. curcas) is a plant that has numerous medicinal and industrial applications. Evaluation of in vitro and in vivo acute/sub chronic hepato-renal toxicity of crude methanol J. curcas leaf extract on albino rats, in vitro toxicity of the fractions and characterization of these fractions were carried out in this study. Study Design: Twenty albino rats were grouped into four, orally administered; 0, 100, 200, and 250 mg/kg daily of the crude extract for 28 days. Place and Duration of Study: Department of Biochemistry, Bayero University Kano and Department of Pure and Applied Chemistry, Bayero University Kano between July 2016 and April 2017. Original Research Article