Advances in Animal and Veterinary Sciences June 2023 | Volume 11 | Issue 6 | Page 910 INTRODUCTION C orn is a primary source of energy for livestock and a major raw material in poultry feed, accounting for up to 50% of the ration. Yellow corn is particularly suitable for poultry feed because it contains essential amino acids, high energy content, and is a source of provitamin A, specifically carotenoids (Edi, 2021; Gupta et al., 2019). However, the use of corn as food and biofuels competes fiercely with its usage as animal feed, making it one of the most expensive commodities in the market. erefore, it is necessary to identify alternative feed ingredients that are readily available, cost-effective, and have nutritional profiles nearly identical to corn. Local feed ingredients from agro-industrial waste, such as cassava and tofu dregs, have great potential as poultry feed ingredients but are rarely employed in Indonesia (Khasanah et al., 2022). Cassava has high energy content similar to corn but low crude protein and high crude fiber, while Research Article Abstract | is research aims to increase the sustainability of feed availability by creating feed independence. Corn is the primary energy source for livestock, but when the price of corn rises and its supply declines, it becomes essential to find alternative ingredients to use in animal feed. is study explores the use of cassava (tapioca by-products) and tofu dregs as substitutes for corn through fermentation technology using Neurospora sp. is produces alternative feed ingredients with nutrient levels that are in balance with corn. To improve the quality of the cassava-tofu dreg mixture, this study used red oncom mushrooms enriched with Na-glutamate during the fermentation process. e research used a completely randomized factorial design, where two factors were considered: the addition level of Na-glutamate (0%, 0.5%, 1%, and 1.5%), and the addition level of red oncom mushrooms (0%, 5%, 10%, and 15%). Each treatment was repeated twice, followed by an orthogonal polynomial test. e variables observed were nutrient contents, including moisture, crude fat, crude protein, crude fiber, NFE, calcium and phosphor, and anti-nutritional phytic acid. e results showed that the fermentation process using red oncom mushrooms enriched with Na-glutamate improved the nutritional level of the cassava-tofu dreg mixture. Although the NFE content decreased, the crude fiber content increased, and the amounts of calcium, phosphorus, and protein significantly increased (P < 0.05). Furthermore, fermentation reduced the phytic acid content to 90.83% at 1.5% Na-glutamate and 15% red oncom mushroom level. In conclusion, this research demonstrates that the quality of cassava-tofu dreg mixture can be enhanced through fermentation using red oncom mushrooms enriched with Na-glutamate. is approach can increase the sustainability of feed availability and reduce the dependence on corn as the primary energy source for livestock. Keywords | Alternative feed, Cassava dreg, Fermentation, Red oncom mushrooms, Tofu dreg MUNASIK*, TITIN WIDIYASTUTI, CARIBU HADI PRAYITNO Corn Substitution rough Na-Glutamate and Neurospora Species Supplementation in Cassava and Tofu Dregs Received | March 16, 2023; Accepted | January 31, 2023; Published | May 03, 2023 *Correspondence | Munasik, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, 53122, Indonesia; Email: munasik@unsoed.ac.id Citation | Munasik, Widiyastuti T, Prayitno CH (2023). Corn substitution through na-glutamate and neurospora species supplementation in cassava and tofu dregs. Adv. Anim. Vet. Sci. 11(6):910-918. DOI | https://dx.doi.org/10.17582/journal.aavs/2023/11.6.910.918 ISSN (Online) | 2307-8316 Copyright: 2023 by the authors. Licensee ResearchersLinks Ltd, England, UK. is article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY ) license (https://creativecommons. org/licenses/by/4.0/). Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, 53122, Indonesia.