Open Access Research Article Journal of Clinical Immunology and Microbiology Ha VTT | Volume 3; Issue 1 (2022) | JCIM-3(1)-041 | Research Article Citation: Vo TTB, et al. Analysis of Antibiotic Resistance Genes of the Extensively Drug Resistant Clinical Klebsiella pneumonia Isolates in Vietnam. J Clin Immunol Microbiol. 2022;3(1):1-15. DOI: http://dx.doi.org/10.46889/JCIM.2022.3101 Analysis of Antibiotic Resistance Genes of the Extensively Drug Resistant Clinical Klebsiella pneumonia Isolates in Vietnam Van Thi Thu Ha 1,2 , Hieu Duc Nguyen 1 , Minh Ngoc Nghiem 1 , Son Thai Nguyen 2 , Tram Bao Nguyen 1 , Duc Minh Nguyen 1 , Cuong Xuan Hoang 3 , Thuy Thi Bich Vo 1* 1 Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, Vietnam 2 Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam 3 Vietnam Military Medical University, Hanoi, Vietnam * Corresponding Author: Thuy Thi Bich Vo, Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, Vietnam; Email: thuytbvo@igr.ac.vn Received Date: 05-02-2022; Accepted Date: 21-02-2022; Published Date: 28-02-2022 Copyright © 2022 by Vo TTB, et al. All rights reserved. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Abstract Background: The over using of antibiotics in hospitals, communities and agriculture has raised drug resistance in Vietnam. Objective: The study was to identify the multi antimicrobial resistance gene expression associated with extensively drug resistance phenotype in Klebsiella pneumonia (K.pneumoniae) isolated from clinical samples in the Military Hospital 103 in Vietnam. Material and Method: Seventeen extensively drug resistant (XDR) strains were identified and measured antibiotic susceptibility by using Vitek 2 Compact System. Genes related to the antimicrobial resistant were screened using in-house multiplex PCR at Vietnam Institute of Genome Research. Results: K.pneumoniae showed resistance to almost fifteen antibiotics with very high rates of over 70%, only sensitive to colistin 100%. There is a correlation between carpabenem resistance and XDR status in the K.pnemoniae group. Most of the K.pneumoniae isolates harbored the widely distributed ESBL gene groups. All the isolates carried more than 3