89 Introduction The Earths surface consists of 70% water, which inhabited by 80% of all the life forms and consequently aquatic life have a greater diversity than their terrestrial counterparts. Marine environment exploration for research is still in early phase, many mysteries associated with aquatic flora and fauna have yet to be discovered. Hence it is very much an important area of research to many investigators to explore rich biodiversity. Despite being comprised of a diverse ecosystem, the marine habitat is rich with nutrients and metabolite and the challenge for the investigators to access and culture most of the bacteria in laboratory conditions to study their metabolic activities. Heterotrophic bacteria play a significant role in the biogeochemical cycle of carbon and other material such as nitrogen and sulphur in the ocean because of their high ISSN: 2347-3215 Volume 2 Number 4 (April-2014) pp. 89-93 www.ijcrar.com ABSTRACT Microbes account for all known life forms for nearly 50 to 90% of Earths history-Life itself began in the ocean. Marine microbes play significant role in the Earth system. Among the microbes bacteria are most vital link directly and indirectly with human life. In view of these total three sites along Vasai coast which is a part of Arabian Sea chosen viz.kalamb, Rangaon and Bhuigaon sea sites were selected. From these area total 227 bacterial isolated were collected by standard isolation procedure and then the characterization with respect to cultural and morphological characters were recorded. Among these the ratio of Gram positive and Gram negative were found to be varied as the dominance of Gram negative was comparatively more in all the three sites. Among the pigmented colonies yellow, red, pink, brown, white, cream etc. are preserved .The pigmentation and incubation temperature has co- relation as pigmentation was observed at incubation temperature 25 0 -28 0 C. . KEYWORDS Marine bacteria, pigmentation, Gram positive, negative, Arabian sea, isolates etc. Isolation of Marine Bacteria along Vasai Coast (M.S.) India Mukesh.R.Pimpliskar* and Rahul.N.Jadhav Vidyavardhinis, E.S.A.college of Science, Vasai Road,Dist-Thane, India *Corresponding author