~ 1504 ~  International Journal of Chemical Studies 2017; 5(6): 1504-1506                     P-ISSN: 2349–8528  E-ISSN: 2321–4902 IJCS 2017; 5(6): 1504-1506 © 2017 IJCS Received: 12-09-2017 Accepted: 14-10-2017   Parvesh Devi Deptartment of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India Sushila Singh Deptartment of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India Promila Deptartment of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India Correspondence Parvesh Devi Deptartment of Chemistry and Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India Isolation and characterization of chemical constituents from Dalbergia sissoo Roxb. Stem Parvesh Devi, Sushila Singh and Promila Abstract Dalbergia sissoo is an important medicinal plant and commonly known as sisu, shisham, tahli, jag at different parts of world and belongs to family Fabaceae. D. sissoo plant parts contain a large number of chemical constituents; leaves of plant contain trisaccharides, oligosaccharides, phenols and the flower of shisham contains tectorigenin biochanin. Stem-bark contains flavonoids, dalbergichromene cinnamylphenols, 4-phenylchromene. Root-bark contains chalcone, isoflavone biochanin A, flavone, retenoid, dehydroamorphigenin. Methanolic extract of D. sissoo stem was mixed with silica gel (60-120 mesh) and subjected to column chromatography to carry out isolation of the compounds from stem of D. sissoo. Chromatographic separation was carried out over silica gel (60-120 mesh) column and eluted with the solvents of increasing polarity. The column chromatography afforded three compounds suchas D- 1(Tritriacontane), D-2 (Dalbergichromene) and D-3 (1,6-Dihydro-1,6-dihydroxy-4-methoxydalbergione). Keywords: Dalbergia sissoo, Fabaceae, Tritriacontane, Dalbergichromene and 1,6-Dihydro-1,6- dihydroxy-4-methoxydalbergione Introduction Natural plant products are easily degradable, less persistent in the environment and eco- friendly in nature. Natural products obtained from various plant materials including terrestrial plants play an important role in chemistry of drugs [1] . They provide amazing source of new drugs leading to development of new chemical entities for further drug development. The scope, importance and methodology of this field have been out lined by various workers [2, 3] . From the human civilization, medicinal plants have been the part and parcel of human society to combat disease. D. sissoo is an important medicinal plant and commonly known as sisu, shisham, tahli, jag at different parts of world and belongs to family Fabaceae. It is native to the Indian subcontinent and southern Iran. It is found in India, Pakistan, Burma, Sri Lanka and Mauritius. The genus Dalbergia consists of 300 species out of which 25 species occur in India and the most famous species from these are the rosewoods [4] . D. sissoo is a medium to large size tree of about 25 meters height with grey yellow trunk, leaves are leathery and 2-3 meters in diameter, pinnately compound. They are broad, ovate, acuminate, glabrescent, petiolate with finepointed tip [5] . Flowers of Shisham whitish to pink in colour, give fragrant and sessile, they are 5-8 mm long racemes, 2.5 - 3.7cm long in short axillary panicles and its crown in oval shape and pods are oblong, flat, thin, strap like 4-8cm long and cm wide with 1-4 seeds. Seeds are 4-5 mm size and kidney shaped, thin and flat, light brown. They have long taproot system and numerous surface roots which produce suckers. The sapwood is white to pale brown in colour. The flowering period of plant is March-May. D. sissoo plant parts contain a large number of chemical constituents; leaves of plant contain trisaccharides, oligosaccharides, phenols, neoflavenes [6, 7, 8] . The flower of shisham contains tectorigenin biochanin. Stem-bark contains flavnoids, dalbergichromene cinnamylphenols, 4-phenylchromene [9, 10, 11] . Root-bark contains chalcone (2, 3-dimethoxy-4′-γ, γ-dimethylallyloxy-2′-hydroxychalcone), isoflavone (7-γ, γ-dimethylallyloxy-5-hydroxy-4′-methoxyisoflavone), biochanin A, flavone, 7-hydroxy- 6-methoxyflavone, retenoid, dehydroamorphigenin [12] . Materials and Methods The D. sissoo stem were collected from CCS, Haryana Agricultural University grounds, Hisar. Chemicals: The economically accessible chemicals from Sigma Aldrich, Qualigens, Merk and Ranbaxy, of high virtue, were utilized for different exploratory methodology.