Scientific Journal of Kurdistan University of Medical Sciences No.120/ July-sep 2022/1-11 Association of rs1800624 Polymorphism in Receptor for Advanced Glycation End Products Gene Promoter with the Risk of Diabetic Nephropathy Iman Salahshourifar 1 , Abbas Tavakoli 2 , Navid Mohammadi 3 , Elham Hajialilo 4 , Hossein Piri 5 1. Assistant professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. ORCID ID: 0000-0001-9987-1188 2. MSc student, Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran. ORCID ID: 0000-0002-2965-3983 3. Professor, School of medicine, children's growth research center, Qazvin University of Medical Sciences, Qazvin, Iran. ORCID ID: 0000-0002-0534-0763 4. Assistant professor, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran. ORCID ID: 0000-0003-2159-4066 5. Associate Professor, Cellular and Molecular Research Center, Research Institute for prevention of Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran. (Corresponding Author), Tel: +98-283-3336001-6, Email: hosseinpiry@gmail.com. ORCID ID: 0000-0001-8402-7742 ABSTRACT Background and Aim: Although the molecular mechanisms involved in the pathogenesis of diabetic nephropathy are still unclear, the role of advanced glycation end products (AGEs) and their associated receptors (AGER) in initiating the inflammatory process in this disease has attracted attention. The aim of this study was to investigate the relationship between rs1800624 polymorphism of AGER gene with risk of diabetic nephropathy in Iranian population. Materials and Methods: In this case-control study, patients were divided into two groups, group1 without diabetic nephropathy (n = 71) and group2 with diabetic nephropathy (n = 79). TETRA-Primer ARMS-PCR technique was used to determine the frequency of genotype and allele of rs1800624 polymorphism in the promoter region of AGER gene. Using standard methods, biochemical tests including measurement of glucose, creatinine, glycosylated hemoglobin and blood urea nitrogen and calculation of eGFR were performed. We used SPSS and FAMHAP softwares for data analysis. Results: The results showed that AA genotype rs1800624 polymorphism in the promoter region of the AGER gene may be associated with an increased risk of diabetic nephropathy. Allele analysis also showed that allele A of the polymorphism may be associated with an increased risk of developing nephropathy, although the results were not statistically significant between the two groups in relation to rs1800624 polymorphism. Conclusion: The findings of this study showed that there was no statistically significant relationship between rs1800624 polymorphism in AGER gene with diabetic nephropathy in the Iranian population, but increase in sample size may result in a tendency to develop diabetic nephropathy. Keywords: Diabetic nephropathy, AGER gene, Polymorphism, Iranian population Received: Aug 23, 2020 Accepted: July 13, 2021 Copyright © 2018 the Author (s). Published by Kurdistan University of Medical Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBYNC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal How to cite the article: Iman Salahshourifar, Abbas Tavakoli, Navid Mohammadi, Elham Hajialilo, Hossein Piri. Association of rs1800624 Polymorphism in Receptor for Advanced Glycation End Products Gene Promoter with the Risk of Diabetic Nephropathy.SJKU 2022;27(3):1-9. [ DOI: 10.52547/sjku.27.3.1 ] [ Downloaded from sjku.muk.ac.ir on 2023-03-02 ] 1 / 11