Anti- SARS- CoV- 2 activity of Andrographis paniculata extract and its major component Andrographolide in human lung epithelial cells and cytotoxicity evaluation in major organ cell representatives Khanit Sa-ngiamsuntorn 1,# , Ampa Suksatu 2,# , Yongyut Pewkliang 3 , Piyanoot Thongsri 3 , Phongthon Kanjanasirirat 4 , Suwimon Manopwisedjaroen 2 , Sitthivut Charoensutthivarakul 4,5,6 , Patompon Wongtrakoongate 6,7 , Supaporn Pitiporn 8 , Phisit Khemawoot 9 , Somchai Chutipongtanate 9,10 , Suparerk Borwornpinyo 4,11,* , Arunee Thitithanyanont 2,* Suradej Hongeng 4,10 1 Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand 2 Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand 3 Section for Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand 4 Excellent Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand 5 School of Bioinnovation and Bio-Based Product Intelligence, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 6 Center for Neuroscience, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 7 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 8 ChaoPhya Abhaibhubejhr Hospital, Prachin Buri 25000, Thailand 9 Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samutprakarn 10540 Thailand 10 Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand 11 Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand # These authors contributed equally to this work * Shared co-corresponding authors: Corresponding authors: Suparerk Borwornpinyo, Ph.D. Excellence Center for Drug Discovery (ECDD), Faculty of Science, Mahidol University, Bangkok 10400 Thailand. Tel: +66-2-201-5308 Email: bsuparerk@gmail.com Arunee Thitithanyanont, M.D. Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand. Tel: +66-2-201-5528 Email: arunee.thi@mahidol.edu . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.08.415836 doi: bioRxiv preprint