RESEARCH ARTICLE Sea grapes extract improves blood glucose, total cholesterol, and PGC-1α in rats fed on cholesterol- and fat- enriched diet [version 2; peer review: 1 approved, 1 approved with reservations] Mury Kuswari 1 , Fahrul Nurkolis 2 , Nelly Mayulu 3 , Faisal Maulana Ibrahim 4 , Nurpudji Astuti Taslim 5 , Defny Silvia Wewengkang 6 , Nindy Sabrina 7 , Ghafur Rasyid Arifin 8 , Keren Esther Kristina Mantik 3 , Muhammad Rahimi Bahar 9 , Najda Rifqiyati 2 , Ronald Rompies 9 , Piko Satria Augusta 10 , Happy Kurnia Permatasari 10 1 Nutrition, Universitas Esa Unggul, Jakarta, Jakarta, 11510, Indonesia 2 Biological Sciences, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga Yogyakarta), Yogyakarta, Yogyakarta, 55281, Indonesia 3 Nutrition, Sam Ratulangi University, Manado, North Sulawesi, 95115, Indonesia 4 Pharmaceutical Analysis and Medicinal Chemistry, Universitas Padjajaran, Sumedang, West Java, 45363, Indonesia 5 Nutritional Sciences, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia 6 Pharmacy, Sam Ratulangi University, Manado, North Sulawesi, 95115, Indonesia 7 Nutrition, Sahid University of Jakarta, South Jakarta, Jakarta, 12870, Indonesia 8 Medicine, University of Indonesia, Depok, West Java, 16424, Indonesia 9 Pediatrics, Sam Ratulangi University, Manado, North Sulawesi, 95115, Indonesia 10 Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia First published: 02 Aug 2021, 10:718 https://doi.org/10.12688/f1000research.54952.1 Latest published: 13 Sep 2021, 10:718 https://doi.org/10.12688/f1000research.54952.2 v2 Abstract Background: Sea grapes or Caulerpa racemosa have a lot of phytochemical content, especially unsaturated fatty acids that are beneficial for health. This study aims to evaluate the effects of sea grapes extract on blood glucose levels, total cholesterol-, and Peroxisome proliferator-activated receptor-gamma coactivator (PGC)- 1α in male Wistar rats, which were given per-oral (p.o.) cholesterol- and carbohydrates fat-enriched diets (CFED). Methods: Forty male Wistar albino rats weighing between 200 – 250 g were used for this study. Animals were randomly distributed into four groups of ten animals each. Group A served as control (received standard dry pellet diet). Rats in group B were fed on CFED for 4 weeks. Groups C and D were fed on CFED and were administered 150 and 450 mg/kg of sea grapes extract (p.o.), respectively. Results: Group C rats indicated a blood glucose reduction and an increase in PGC-1α serum, in Open Peer Review Reviewer Status Invited Reviewers 1 2 version 2 (revision) 13 Sep 2021 report report version 1 02 Aug 2021 report Carla F. Kairupan, Universitas Sam Ratulangi, Manado, Indonesia 1. Page 1 of 15 F1000Research 2021, 10:718 Last updated: 08 NOV 2021