cognizancejournal.com Ali A. Sabi, Cognizance Journal of Multidisciplinary Studies, Vol.4, Issue.12, December 2024, pg. 575-584 (An Open Accessible, Multidisciplinary, Fully Refereed and Peer Reviewed Journal) ISSN: 0976-7797 Impact Factor: 4.843 Index Copernicus Value (ICV) = 77.57 ©2024, Cognizance Journal, ZAIN Publications, Fridhemsgatan 62, 112 46, Stockholm, Sweden, All Rights Reserved 575 Synthesis and Characterizing a Novel Polyamide to Inhibit Replication of Viral Protein inside RNA Molecule Ali A. Sabi Department of Pharmaceutical Chemistry, College of Pharmacy, University of Kufa, Najaf, Iraq DOI: 10.47760/cognizance.2024.v04i12.052 Abstract: N-methyl pyrrole/N-methyl imidazole hairpin polyamides are small aromatic rings that typically select the minor groove of DNA and make the DNA molecule more stable. Hairpin polyamides can recognize the minor groove of DNA without exposing it to denaturation. Polyamides (PAs) have gained interest because of arranging gene expression, inhibiting vesicular stomatitis virus (VSV) replication, and their role against human papillomavirus HPV. Since the structure of VSV most likely is an identity RNA duplex in one strand, it has been selected as a prototype virus to test the potential of polyamides for inhibition. Among six sequences of polyamide, only one molecule numbered 1011 showed the ability to control VSV. Based on this fact, we synthesized a novel polyamide that resembles 1011 in its building blocks with an exchange pyrrole ring by imidazole to enhance the interaction between polyamide and genomic protein of VSV. All negative-strand RNA viruses have the same RNA structure in the nucleocapsid and that makes the polyamides a good target for negative-strand RNA viruses, such as respiratory syncytial virus and Ebola virus. KEYWORDS CSBio synthesizer, antiviral agents, negative-strand RNA virus, nucleocapsid, peptide synthesizer, polyamide RNA synthesis, solid-phase. Introduction VSV is a negative-strand RNA virus, and its genomic RNA forms a single-stranded structure, which is encapsidated by nucleoproteins. Vesicular stomatitis virus (VSV), and Respiratory syncytial virus (RSV) are famous viruses that belong to the family of negative-strand RNA viruses (NSVs).[1] These pathogenic viruses can cause acute infections in infants and young children. RSV was isolated in early 1956, and soon afterward it was available again for isolation in children to become globally known as a severe virus-infected infant.[2]Furthermore, RSV is a type of enveloped virus that had led to more than 30 million new episodes in children under five years with severe hospitalization cases in 2005.[3] Treatment of this virus requires an active drug, and no effective vaccine is available therefore the need for novel antiviral medications has gained priority from researchers worldwide. Also, no effective antiviral drugs are available for many deadlier negative-strand RNA viruses such as vesicular stomatitis, rabies,