Suitability of the electrical conductivity test and antioxidant enzyme activity to assess the vigor of lentil (Lens culinaris Medik.) seeds Adequação do teste de condutividade elétrica e atividade de enzimas antioxidantes para avaliar o vigor de sementes de lentilha (Lens culinaris Medik.) Marcelo Augusto Rocha Limão 1 *; Denise Cunha Fernandes dos Santos Dias 2 ; Gabriel Cordeiro de Oliveira Peris 3 ; Ana Clara Reis Trancoso 4 ; Rafaela Marques de Miranda 4 ; Warley Marcos Nascimento 5 Highlights There is a demand for fast and efficient vigor tests for lentil seeds. These tests are relevant to speed up lot quality control. The electrical conductivity test is efficient for classifying the vigor of lots. The activity of antioxidant enzymes is related to physiological potential. Abstract Adapting methodologies that enable the assessment of lentil seed vigor is of utmost importance to ensure the marketing of lots with outstanding field performance. This study aimed to define the appropriate conditions for conducting the electrical conductivity test for lot classification according to vigor and assess whether the activity of antioxidant enzymes is related to the physiological potential of lentil seeds. For this, seeds from seven lots of the cultivar Silvina were subjected to different tests to characterize their initial quality, and the results were compared with those obtained in the electrical conductivity test and enzyme activity. Four soaking periods (4, 8, 12, and 24 h) associated with three water volumes (75, 100, and 150 mL) at 25 °C were used to assess electrical conductivity. The SOD, CAT, and APX enzymes were also assessed. The electrical conductivity test and enzyme activity were efficient in assessing the physiological potential with information related to other vigor tests. The electrical 1 Doctoral Student in Phytotechnics, Postgraduate Program in Phytotechnics, Universidade Federal de Viçosa, UFV, Viçosa, MG, Brazil. E-mail: marceloliimao@gmail.com 2 Prof a Dr a at the Department of Agronomy, UFV, Viçosa, MG, Brazil. E-mail: dcdias@ufv.br 3 Agronomy Undergraduate Student, Department of Agronomy, UFV, Viçosa, MG, Brazil. E-mail: gabriel.peris@ufv.br 4 PhD. In Phytotechnics, Postgraduate Program in Phytotechnics, UFV, Viçosa, MG, Brazil. E-mail: anaclara.trancoso@ gmail.com; rafaela.ouropreto@yahoo.com.br 5 Researcher, Brazilian Agricultural Research Company - Vegetables, EMBRAPA, Brasilia, DF, Brazil. E-mail: warley. nascimento@embrapa.br * Author for correspondence 799 Semina: Ciênc. Agrár. Londrina, v. 45, n. 3, p. 799-818, maio/jun. 2024 DOI: 10.5433/1679-0359.2024v45n3p799 Received: Feb. 19, 2024 Approved: May. 07, 2024 ARTICLES / ARTIGOS