Jurnal Riset Biologi dan Aplikasinya, Volume 5, Issue 2, September 2023 Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice Ni Kadek Prederika Sari Andayani 1 , Iriani Setyawati 2* , Ni Wayan Sudatri 3 1 Alumni of Biology Study Program, Faculty of Matematics and Natural Sciences, Udayana University, Bukit Jimbaran Campus, Badung, Bali. 2 *Biology Study Program, Faculty of Mathematics, Natural and Earth Sciences, Universitas Negeri Manado, Tondano Campus, North Sulawesi. 3 Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University, Bukit Jimbaran Campus, Badung, Bali. * Corresponding author e-mail: irianisetyawati@unima.ac.id Article History ABSTRACT Received : 11 May 2023 In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum. Revised : 12 July 2023 Accepted : 11 September 2023 Published : 30 September 2023 Keywords Digestive tract, herbal medicine, histopathology, M. pudica How to cite: Andayani, N.K.P.S., Setyawati, I., & Sudatri, N.W. (2023). Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice. Jurnal Riset Biologi dan Aplikasinya, 5(2):52-59. DOI: 10.26740/jrba. v5n2.p.52-59 INTRODUCTION Indonesia has various kinds of herbal plants that are efficacious for healing diseases. About 10% of all plants in Indonesia are predicted to be medicinal plants. At least 80% of species of medicinal plants in Southeast Asia, whether native or introduced, can be found in Indonesia. (Cahyaningsih et al., 2021). Although many types of plants have the potential to be used as herbal medicines, most have not been tested and have not been monitored at all. The problem is the lack of knowledge about the mechanism, the potential for adverse reactions, contraindications, and interactions with other drugs used and with the food consumed. Safety continues to be a major issue in the use of herbal medicines; therefore, research and testing are needed to ensure that all herbal medicines are safe and of good quality (Ekor, 2014). Herbal plants are now widely circulated in the market and are used as alternative medicines for disease recovery processes. One of the plants that has long been used by the people of Indonesia as a medicinal ingredient is M. pudica Linn, from the Fabaceae family. According to Ahmad et al. (2012), all parts of this plant have the potential to have medicinal properties. In India, the M. pudica plant is cultivated because of its many benefits and is very useful for the people of India, as a vegetable, spice, cosmetic oil, and medicinal plant. Jurnal Riset Biologi dan Aplikasinya https://journal.unesa.ac.id/index.php/risetbiologi