INTRODUCTION One type of coughing is triggered by mechanical stimulation, whereas chemicals, including sulphur dioxide, ammonia, citric acid, and capsaicin, trigger the other type. Some evidence suggests that cough suppressants work best when used to temporarily alleviate symptoms. The anti-tussive effect is just one of several conditions for which herbal medicines are increasingly being employed as a treatment. 1 Cough suppressants are available in a variety of forms and are frequently used in combination. Before getting into the special type of medicine used, it's essential to look more carefully at nature of coughing, its function in disease, and the benefits of suppressing it. Since prehistoric times, people have used traditional medicine derived from plants to treat a broad variety of illnesses. Early humans realized their need for nature in both good health and unhealthy lifestyles. Throughout history, nature has served as a valuable reservoir of medicinal resources, with numerous contemporary pharmaceuticals being derived from traditional healing traditions. Numerous contemporary pharmaceuticals have been derived from natural origins, with plants being particularly noteworthy in this regard. The application of medicinal chemistry, combinatorial chemical techniques, and biosynthetic technology will conduct the optimisation of novel natural product leads. This approach aims to develop chemotherapeutic agents and other bioactive medications that demonstrate enhanced efficacy. The popularity of alternative treatments, particularly herbal medicine, has skyrocketed in recent decades. Piper longum Linn The fruits contain approximately 1% of volatile oil, resin, alkaloids such as piperine and piperlonguminine, a waxy alkaloid known as nisobutyldeca-trans-2-trans-4-dienamide, and a terpenoid compound. The predominant factor contributing to the fruit's tart flavor is mostly attributed to the presence of piperine, a piperidine alkaloid. In addition to their nutritional composition, the fruits are found to include calcium (1230 mg/100 g), phosphorus (190 mg/100 g), and iron ABSTRACT The l-phellandrene and caryophyllene found in Piper longum fruits are effective against a wide range of infections, including stomachic, bronchitis, spleen, cough, tumor, and asthma. This study reports preparing and characterizing alcohol-soluble extract of P. longum L. fruit powder. FTIR spectroscopy and TLC were used to characterize an alcoholic extract of P. longum. P. longum L. alcohol soluble extract was tested for its anti-tussive efficacy using the standard mouse cough model caused by ammonia liquor. Cough frequency was inhibited by 49.51% (Marketed formulation), 50.84% (Extract 100 mg/kg), 62.71% (Extract 200 mg/kg). Statistically significant (p < 0.001) difference was observed in %inhibition of cough frequency of both doses of extract when compared with the marketed formulation. This study reveals an anti-tussive activity of alcohol soluble extract of P. longum L. Keywords: Alcohol soluble extract, Anti-tussive activity, Piper longum Linn, Total Phenolic content. International Journal of Pharmaceutical Quality Assurance (2023); DOI: 10.25258/ijpqa.14.3.53 How to cite this article: Bedse A, Nalawade A, Kamandar P, Wagh A, Mahajan K, Raut S, Dhamane S. Antitussive Activity of Alcoholic Extract of Piper longum Linn. International Journal of Pharmaceutical Quality Assurance. 2023;14(3):771-775. Source of support: Nil. Conflict of interest: None Antitussive Activity of Alcoholic Extract of Piper longum Linn. Anjali Bedse 1 , Ashwini Nalawade 2 , Pallavi Kamandar 3 , Anita Wagh 4 , Komal Mahajan 5 , Shilpa Raut 6 , Suchita Dhamane 7 * 1,6 Department of Pharmaceutics, K.K.Wagh College of Pharmacy, Nashik, Affiliated to Dr. Babasaheb Ambedkar Technological University, Lonere, Raigad, Maharashtra, India. 2,7 Department of Pharmaceutics, Jayawantrao Sawant College of Pharmacy and Research, Hadapsar, Pune, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India. 3 Department of Pharmaceutics, Institute of Chemical Technology Jalana, Maharashtra, India. 4 Department of Pharmacognosy, Srinath College of Pharmacy, Aurangabad, Affiliated to Dr.Babasaheb Ambedkar Technological University, Lonere, Raigad, Maharashtra, India. 5 Department of Pharmacology, K. K. Wagh College of Pharmacy, Nashik, Dr. Babasaheb Ambedkar Technological University, Lonere, Raigad, Maharashtra,India. Received: 12 th April, 2023; Revised: 18 th July, 2023; Accepted: 22 th August, 2023; Available Online: 25 th September, 2023 RESEARCH ARTICLE *Author for Correspondence: spd.jscopr@gmail.com