This is the final peer-reviewed accepted manuscript of: Lazzaro, D, Morigi, S, Melpignano, P, Loli Piccolomini, E, Benini, L. Image enhancement variational methods for enabling strong cost reduction in OLED-based point-of-care immunofluorescent diagnostic systems. Int J Numer Meth Biomed Engng. 2018; 34:e2932. The final published version is available online at https://doi.org/10.1002/cnm.2932 . Rights / License: The terms and conditions for the reuse of this version of the manuscript are specified in the publishing policy. For all terms of use and more information see the publisher's website.