International Journal of Membrane Science and Technology, 2023, Vol. 10, No. 4, pp 2322-2329 2322 In-Silico Screening of Phytocompounds of Justicia Adhatoda for Thrombolytic Activity V. Poorna Pushkala 1* , H. Subhashree 2 , K. Yazhini 2 1* Professor, Department of Microbiology, Sri Sairam Siddha Medical College & Research Centre, Chennai, Tamil Nadu, India. poorna.mku@gmail.com 2 Third profession BSMS, Sri Sairam Siddha Medical College and Research Centre, Chennai, India Abstract: Background: The risk of thrombosis and its associated mortality is becoming a greater public health concern in both industrialized and developing nations. Clinical condition that is myocardial infarction, ischemic stroke, unstable angina, peripheral vascular diseases like deep vein thrombosis prioritise top in the list of thrombotic disorders. Conventional thrombolytic medications including alteplase, anistreplase, streptokinase and urokinase offer life risking adverse effects like haemorrhage, stroke, vascular dysfunction, hypertension, internal bleeding and so on. In order to overcome the undesirable side effects caused by the conventional thrombolytic therapy, there is ominous need of alternate complementary treatment from traditional medicine. Majority of the Siddha formulations comprise of some potential medicinal herbs which are found to be biocompatible and also have a wide safety window. Justicia adhatoda is one such novel herb which is investigated broadly by researchers till date, aspired by its unique pharmacological property rendered by the bioactive components present in it. Methods: Hence, the present research is aimed at in- silico screening of certain classes of alkaloids and flavonoids retrieved from J. adhatoda to explore the possible anti- thrombotic activity against the target human plasminogen activation loop peptide using AutoDock screening tool. Results and Conclusion: The computational analysis’s findings led to the conclusion that the bioactive compounds present in Justicia adhatoda like Astragalin, Kaempferol, Vitexin, Vasicolinone and Adhatodine reveals significant binding affinity against target plasminogen. Thereby it was determined that these compounds may have promising anti-thrombotic efficacy due to their considerable binding affinity towards the target plasminogen. Keywords: Siddha, Thrombosis, Thrombolytic agents, Justicia adhatoda, bioactive components, Adhatodine, Anti-thrombotic activity 1. INTRODUCTION Platelet activation is a conventional and spontaneous process in the sequence of blood clotting which is mediated by complex extrinsic and intrinsic pathways. Signals innervated by the injured blood vessels aggravate the mode of platelet adhesion which in turn forms the network of platelet mesh upon stabilization which may form the clot which prevents excess blood loss [1]. Perhaps this event of spontaneous thrombosis without major vascular involvement surely calls for health issues which are collectively called as thrombotic disorders [2]. Due to extensive action caused by thrombolytic drugs (alteplase, anistreplase, streptokinase (SK), urokinase) in thinning the bloods there might be a possible risk of haemorrhage, stroke, hypertension, internal bleeding, vascular dysfunction etc [3]. By considering these serious undesirable effects caused by the conventional thrombolytic agents, the search of exploring alternate remedies preferably from herbal origin grabs greater attention. The Siddha system of medicine that uses herbal therapeutics has proven to heal wide range of diseases in humans for several centuries [4]. Justicia adhatoda is a novel medicinal herb which is largely investigated owing to its unique pharmacological property rendered by its bioactive components. It belongs to the family Acanthaceae. Geographically this class of species is widely distributed in the central zone of South Asia and the Indo-China region [5]. J. adhatoda